FastQCFastQC Report
Wed 25 May 2016
SRR1780845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380476
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT105432.771002638799819No Hit
ATTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT32280.8484109378778162No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21370.5616648619098182No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG19150.5033168977806747No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC10570.2778099012815526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA150.00222587869.9831828
GCCGTCA501.4551915E-1163.00142346
ATATCGT1000.059.50134337
AGGGGGG20650.058.15983670
CGTCTAC252.3856286E-456.0159948
ACGTATG252.3902829E-455.993940
GACACGA750.055.98654626
ACGATAT950.055.2571434
CCGTCTT24350.053.7589147
CGTCTTC24650.053.68672648
GCCGTCT24750.053.59716846
TATGCCG25000.053.46714843
CGTATGC25500.053.24910441
ATCGTAT1250.053.201239
ATCTCGT23900.053.01374437
TCGTATG25950.053.0000140
ATGCCGT25300.052.98538644
TGCCGTC25450.052.9481545
GATCTCG24250.052.8259336
TCTTCTG24900.052.7258950