FastQCFastQC Report
Wed 25 May 2016
SRR1780844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282664
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223427.90408400079246No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG15380.544108906687799No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA207.908876E-570.0168151
GCACCTT150.002221047270.0168151
CCAAACG252.3871972E-456.00353615
TACGCTT200.00694490452.503325
GTCGTTT200.00694490452.5033215
CGTTTGC200.00694490452.5033217
GACCTTC305.864891E-446.6778761
CTCGGTG2300.045.64698444
TCGCCGT2250.045.11396452
TCGGTGG2300.044.1254245
TGGTCGC2300.044.1254249
ATCTCGG2400.043.74502642
GATCTCG2400.043.74502641
CGGTGGT2450.042.8522746
GCCGTAT2300.042.6113954
CGCCGTA2450.041.4311953
GTCGCCG2550.041.17907351
CGTATCA2400.040.83591556
TCTCGGT2500.040.59538343
TTGTACT350.001253773340.0025254
Produced by FastQC (version 0.11.5)