FastQCFastQC Report
Wed 25 May 2016
SRR1780844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282664
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT75992.688350833498429No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT53021.8757252426909687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26550.9392777290351796No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG16760.5929301219822829No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC5370.1899782073415787No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC4090.14469476127133274No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT3900.13797299974528063Illumina PCR Primer Index 4 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA550.070.0093848
TCCGCGA150.00222354369.99699463
CGGATTT150.00222354369.99699434
TATTACG150.00222354369.99699425
AGGGGGG16700.060.36736770
TAAAGCG252.3884502E-455.9975958
GCCGAAA252.3884502E-455.9975946
GCATGTT252.3884502E-455.9975919
GTAAAAC252.3884502E-455.9975935
ATTATAC11150.054.0173841
AATCTCG23250.052.6859136
CTCGTAT23650.052.53474439
ATCTCGT23200.052.49774637
ATTACGG200.006947822452.49774626
TACAGCC200.006947822452.4977464
TGCCGAC608.367351E-1152.49774645
TATGCCG23350.052.46027443
TCTCGTA23900.052.42452638
CGTATGC23950.052.31507541
TCGTATG24450.052.24724640