FastQCFastQC Report
Wed 25 May 2016
SRR1780843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30552
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1610652.716679759099236No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG16025.243519245875883No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT1830.5989787902592302No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1190.3894998690756743No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC1070.3502225713537575No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG470.15383608274417387No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCAAGTGTAGATCTCGGTG430.14074365017020163No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG420.13747054202670855No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390.12765121759622938No Hit
CTTATACACATCTGACGCTGCCGACGAGCGAACTAGTGTAGATCTCGGTG380.12437810945273632No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360.1178318931657502No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340.11128567687876408No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330.108012568735271No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC150.002204175370.046
AAGCTTA150.002204175370.045
CGGTGGT2450.054.28571746
AAAAAGG1500.053.66666870
TGGTCGC2350.053.61701649
GGTGGTC2350.053.61701647
GTGGTCG2350.053.61701648
GTCGCCG2400.052.551
GTATCAT2400.052.557
TCGCCGT2400.052.552
CGTATCA2400.052.556
CTCGGTG2400.052.544
GATTACT200.006887761452.51
GGTCGCC2400.052.550
CCGTATC2450.051.42857455
TCTCGGT2450.051.42857443
TATCATT2450.051.42857458
GCCGTAT2450.051.42857454
CGCCGTA2450.051.42857453
TCGGTGG2600.051.1538545
Produced by FastQC (version 0.11.5)