##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30552 Sequences flagged as poor quality 0 Sequence length 76 %GC 79 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.626898402723224 32.0 27.0 32.0 13.0 32.0 2 28.566673212882954 32.0 27.0 32.0 13.0 32.0 3 28.379091385179365 32.0 27.0 32.0 13.0 32.0 4 27.700739722440428 32.0 27.0 32.0 13.0 32.0 5 28.327965435978005 32.0 27.0 32.0 13.0 32.0 6 32.32688531029065 37.0 32.0 37.0 13.0 37.0 7 32.074234092694425 37.0 32.0 37.0 13.0 37.0 8 32.302729772191675 37.0 32.0 37.0 13.0 37.0 9 32.45037968054464 37.0 32.0 37.0 13.0 37.0 10-11 32.01993322859387 37.0 32.0 37.0 13.0 37.0 12-13 31.833529719821943 37.0 32.0 37.0 13.0 37.0 14-15 32.17553678973553 37.0 32.0 37.0 13.0 37.0 16-17 32.6792517674784 37.0 32.0 37.0 13.0 37.0 18-19 32.23928057083006 37.0 32.0 37.0 13.0 37.0 20-21 32.61380597014925 37.0 32.0 37.0 13.0 37.0 22-23 32.67391660120451 37.0 32.0 37.0 13.0 37.0 24-25 31.934701492537314 37.0 32.0 37.0 13.0 37.0 26-27 32.33580452998167 37.0 32.0 37.0 13.0 37.0 28-29 32.49698874050799 37.0 32.0 37.0 13.0 37.0 30-31 32.28413851793663 37.0 32.0 37.0 13.0 37.0 32-33 32.613740507986385 37.0 32.0 37.0 13.0 37.0 34-35 32.086999214454046 37.0 32.0 37.0 13.0 37.0 36-37 32.931919350615345 37.0 32.0 37.0 13.0 37.0 38-39 33.12141594658287 37.0 34.5 37.0 17.5 37.0 40-41 32.55415357423409 37.0 32.0 37.0 13.0 37.0 42-43 32.36086017282011 37.0 32.0 37.0 13.0 37.0 44-45 32.221900366588116 37.0 32.0 37.0 13.0 37.0 46-47 32.432410316836865 37.0 32.0 37.0 13.0 37.0 48-49 32.925324037706204 37.0 32.0 37.0 13.0 37.0 50-51 33.136095836606444 37.0 32.0 37.0 13.0 37.0 52-53 32.3670299816706 37.0 32.0 37.0 13.0 37.0 54-55 32.446451950772456 37.0 32.0 37.0 13.0 37.0 56-57 32.22345509295627 37.0 32.0 37.0 13.0 37.0 58-59 31.74049162084315 37.0 32.0 37.0 13.0 37.0 60-61 31.637225058915945 37.0 32.0 37.0 13.0 37.0 62-63 31.190380335166275 37.0 29.5 37.0 13.0 37.0 64-65 30.938187352710134 37.0 29.5 37.0 13.0 37.0 66-67 31.041093872741556 37.0 27.0 37.0 13.0 37.0 68-69 31.331238544121497 37.0 29.5 37.0 13.0 37.0 70-71 31.43090468709086 37.0 32.0 37.0 13.0 37.0 72-73 31.6889074365017 37.0 32.0 37.0 13.0 37.0 74-75 31.322777559570568 37.0 29.5 37.0 13.0 37.0 76 31.54939120188531 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -6.000669174603637 11101 2 -6.544164600770788 11101 3 -4.415786824294006 11101 4 -4.791347930192515 11101 5 -5.788825591556968 11101 6 -4.336744163857627 11101 7 -4.748971621264172 11101 8 -4.774250836867946 11101 9 -7.274571440796798 11101 10-11 -3.8608144251387024 11101 12-13 -5.673163239662273 11101 14-15 -5.2006352516125745 11101 16-17 -3.614972970016531 11101 18-19 -0.40873961101268463 11101 20-21 -2.1639149208786925 11101 22-23 -2.0553688367443854 11101 24-25 -2.8953069169514585 11101 26-27 -1.768196424205712 11101 28-29 -5.870211453363808 11101 30-31 -4.278167681521637 11101 32-33 -4.516387095896384 11101 34-35 -4.51691494615568 11101 36-37 -4.711925648333924 11101 38-39 -3.631236416177156 11101 40-41 -6.967025672432776 11101 42-43 -4.559760254912064 11101 44-45 -6.231254474668162 11101 46-47 -3.088026489984898 11101 48-49 -4.3167518259559365 11101 50-51 -3.265867048451014 11101 52-53 -8.884512532995394 11101 54-55 -4.669892232566905 11101 56-57 -3.5838761437414277 11101 58-59 -4.86285411116496 11101 60-61 0.5681985634396902 11101 62-63 -7.411356645613861 11101 64-65 -5.840335233276527 11101 66-67 -4.028344282529755 11101 68-69 -7.673028704878337 11101 70-71 -5.293124725668569 11101 72-73 -6.352082573577967 11101 74-75 -5.066578056255633 11101 76 -4.898427291984998 11102 1 1.2147154407809815 11102 2 1.63275847615229 11102 3 -0.6773252858324668 11102 4 0.23172899288440973 11102 5 -0.6503640530954264 11102 6 0.01710198998852519 11102 7 2.7356437633512165 11102 8 0.46421070159359346 11102 9 2.13312086689551 11102 10-11 1.8968778825536035 11102 12-13 0.7268367603377257 11102 14-15 -2.704481405458729 11102 16-17 -0.691896046939604 11102 18-19 1.2104911582180904 11102 20-21 1.2514696945059214 11102 22-23 0.2369388555633094 11102 24-25 1.3854623138177757 11102 26-27 -1.602811808821098 11102 28-29 1.8220962389438853 11102 30-31 -0.11662921998317444 11102 32-33 2.6451513656420786 11102 34-35 -1.4592226384633733 11102 36-37 2.7880743516660758 11102 38-39 2.187994353053618 11102 40-41 1.3368204814133762 11102 42-43 -1.6866833318351375 11102 44-45 1.380283986870296 11102 46-47 0.4042812023227924 11102 48-49 2.9255558663517576 11102 50-51 1.3687483361643729 11102 52-53 1.4808720823892187 11102 54-55 3.0878000751254007 11102 56-57 1.1853546254893388 11102 58-59 0.8371458888350389 11102 60-61 -0.5818014365603084 11102 62-63 -1.7267412609984731 11102 64-65 0.4519724590311682 11102 66-67 0.31396340977793713 11102 68-69 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28-29 0.2686774355250776 11104 30-31 -0.05038990374385577 11104 32-33 2.21139068188139 11104 34-35 0.09419616495543082 11104 36-37 0.4825187961105186 11104 38-39 1.4743191393784016 11104 40-41 -0.03924789465499856 11104 42-43 1.1124619673101606 11104 44-45 -1.0534766968903853 11104 46-47 -1.0658042677626796 11104 48-49 -1.9889740481781573 11104 50-51 0.7341329515489861 11104 52-53 -1.3567347552176159 11104 54-55 0.8356633229886512 11104 56-57 -0.7505428104080956 11104 58-59 -0.2184096667205182 11104 60-61 -0.026245881004751226 11104 62-63 0.3330877988305829 11104 64-65 1.7874425445012534 11104 66-67 -0.489455393640867 11104 68-69 0.2936379617883311 11104 70-71 1.306875274331432 11104 72-73 0.9645840930886962 11104 74-75 0.6611997215221486 11104 76 0.5349060413483357 11105 1 -1.7164586482878441 11105 2 -4.7599540744549955 11105 3 -4.531576297978216 11105 4 -5.586084772297777 11105 5 -3.5046150652411754 11105 6 -3.136744163857628 11105 7 -2.070024252843119 11105 8 -4.811092942131104 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0.7696658177074056 21610 48-49 2.9255558663517576 21610 50-51 2.195671413087446 21610 52-53 0.9231797746969121 21610 54-55 0.5878000751254007 21610 56-57 0.6084315485662657 21610 58-59 0.47176127345042573 21610 60-61 0.45666010190123174 21610 62-63 0.27325873900152686 21610 64-65 1.586587843646555 21610 66-67 1.698578794393324 21610 68-69 -0.5845671664167966 21610 70-71 0.7491829666391254 21610 72-73 1.297917426422032 21610 74-75 4.09496040528283 21610 76 4.54772655416885 21611 1 1.5608692869348246 21611 2 1.2866046299984433 21611 3 0.47652086801368654 21611 4 1.5009597621151798 21611 5 1.7727128699814934 21611 6 3.1324866053731384 21611 7 -1.3797408520334002 21611 8 3.5795953169782067 21611 9 0.8638900976647435 21611 10-11 -0.1415836559079331 21611 12-13 0.11145214495310896 21611 14-15 2.795518594541271 21611 16-17 1.6542577992142427 21611 18-19 1.8258757736027036 21611 20-21 0.2514696945059214 21611 22-23 3.121554240178696 21611 24-25 1.789308467663929 21611 26-27 0.954880498871205 21611 28-29 0.24517316202080508 21611 30-31 0.42183231847836566 21611 32-33 2.1451513656420858 21611 34-35 0.8292388999981668 21611 36-37 0.5380743516660758 21611 38-39 1.5918405068997643 21611 40-41 1.9906666352595295 21611 42-43 -0.3405294856812944 21611 44-45 1.0148993714856829 21611 46-47 2.3658196638612523 21611 48-49 1.0794020201979109 21611 50-51 0.6187483361643729 21611 52-53 3.4424105439276858 21611 54-55 0.5108769982023205 21611 56-57 1.185354625489346 21611 58-59 0.37560742729657903 21611 60-61 2.495121640362772 21611 62-63 2.1578741236169137 21611 64-65 1.0865878436465515 21611 66-67 2.5062711020856305 21611 68-69 2.4923559105062765 21611 70-71 2.518413735869892 21611 72-73 1.259455887960499 21611 74-75 1.9411142514366766 21611 76 1.7784957849380838 21612 1 0.4393308253963646 21612 2 -1.2441646007707874 21612 3 0.46421317570599285 21612 4 0.4286520698074874 21612 5 2.051174408443032 21612 6 -0.37674416385762655 21612 7 1.8310283787358266 21612 8 0.24574916313205364 21612 9 -1.774571440796798 21612 10-11 1.7691855748612966 21612 12-13 -0.41316323966227486 21612 14-15 0.649364748387427 21612 16-17 0.10502702998346791 21612 18-19 1.8812603889873145 21612 20-21 2.116085079121305 21612 22-23 -0.4753688367443871 21612 24-25 0.09469308304854351 21612 26-27 0.6018035757942855 21612 28-29 0.1497885466361879 21612 30-31 -1.3981676815216346 21612 32-33 2.523612904103622 21612 34-35 1.2530850538443232 21612 36-37 0.1680743516660712 21612 38-39 0.6987635838228456 21612 40-41 1.2829743275672172 21612 42-43 1.9702397450879374 21612 44-45 0.7187455253318333 21612 46-47 1.7719735100151013 21612 48-49 -0.06675182595593299 21612 50-51 -0.7158670484510168 21612 52-53 0.5854874670046044 21612 54-55 -0.09989223256690138 21612 56-57 -0.3438761437414257 21612 58-59 1.2571458888350406 21612 60-61 0.6381985634396905 21612 62-63 1.0086433543861375 21612 64-65 -0.2803352332765243 21612 66-67 1.4616557174702471 21612 68-69 2.53697129512166 21612 70-71 1.2268752743314337 21612 72-73 1.2179174264220336 21612 74-75 2.343421943744371 21612 76 0.301572708015005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 11.0 13 35.0 14 57.0 15 105.0 16 165.0 17 269.0 18 325.0 19 377.0 20 394.0 21 456.0 22 495.0 23 521.0 24 543.0 25 635.0 26 720.0 27 828.0 28 931.0 29 1103.0 30 1405.0 31 1622.0 32 2084.0 33 2830.0 34 3614.0 35 4618.0 36 6408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 73.37894013289255 6.0521750515531405 4.4450263493829985 16.12385846617132 2 67.32462601067138 7.11643588988183 18.03659694261678 7.52234115683001 3 67.38027431339815 7.496153720252709 17.539035647648042 7.584536318701103 4 66.60120450379681 13.373919874312648 11.52788688138256 8.496988740507987 5 66.13969625556429 10.483765383608274 18.51924587588374 4.857292484943702 6 70.3283034925207 18.627868154888546 6.353310857255082 4.690517495335668 7 68.9152919612464 10.267740246137732 7.020816967792616 13.796150824823252 8 69.54045561665357 17.815527625032733 6.26472898664572 6.3792877716679754 9 67.80570830060225 6.487300340403247 5.904687090861483 19.80230426813302 10-11 69.42098716941608 12.005760670332547 11.464061272584445 7.109190887666929 12-13 68.6431108128897 8.084709425069146 12.104152005629839 11.168027756411306 14-15 73.68541642799863 10.582131810222084 7.637922851578482 8.09452891020081 16-17 73.1612713168145 8.71166246604039 6.8115609963667305 11.315505220778372 18-19 69.12917532690702 7.610100977038771 11.151661947858534 12.10906174819567 20-21 73.1715300394416 7.90959527355449 8.09943865276664 10.819436034237272 22-23 73.04135775191895 7.690545162926957 7.003158704440189 12.2649383807139 24-25 70.14075286415712 12.034369885433716 6.7921440261865795 11.032733224222586 26-27 73.59583660644148 11.426420528934276 7.23520555119141 7.742537313432836 28-29 73.87732390678188 8.857030636292224 7.389041633935585 9.876603822990312 30-31 73.58110761979576 11.6277166797591 7.23520555119141 7.555970149253731 32-33 69.40462162869862 8.911036920659859 11.531159989526053 10.153181461115475 34-35 69.18053714341828 11.269864650333055 11.98180062519435 7.56779758105432 36-37 73.23775388291517 8.903291271828612 11.998166969444036 5.860787875812179 38-39 73.76560888990721 7.79994435625092 12.3790975893164 6.055349164525473 40-41 73.31554915470598 11.408605142137047 8.893180367576061 6.382665335580905 42-43 69.77284629484159 11.833922492799163 11.658811207122282 6.734420005236973 44-45 68.88645216195869 7.800072010736146 12.554417204019508 10.759058623285654 46-47 72.55855059655325 7.04571119948937 9.168426048673508 11.227312155283874 48-49 74.09904749435371 6.863932440836634 12.146901901738078 6.890118163071585 50-51 77.1766169154229 7.2875752814873005 8.938858339879548 6.596949463210265 52-53 69.45208169677926 7.433228593872741 11.971393034825871 11.143296674522126 54-55 72.5909924063891 7.459413459020686 8.433163131709872 11.516431002880335 56-57 72.52864157119477 7.518821603927988 8.057283142389524 11.895253682487725 58-59 69.18972881855228 11.552624257401437 11.816113775101059 7.441533148945223 60-61 67.99882168106835 8.66555380989788 12.475451688923803 10.860172820109977 62-63 68.13138256087981 12.067949725058916 11.70790782927468 8.092759884786593 64-65 68.27056840313578 12.350043370812262 12.39586913471138 6.983519091340567 66-67 68.3905600471343 16.943798893653238 7.582403194658113 7.083237864554352 68-69 68.24110898348636 17.621642853635784 7.030981489664653 7.106266673213205 70-71 68.67215502708719 17.422543740486752 6.478011096744628 7.427290135681434 72-73 70.07348248040195 16.622751747050064 6.428489599528666 6.875276173019327 74-75 72.60735794710658 13.666863053155275 6.580583922492799 7.145195077245352 76 78.04726368159204 7.8849175176747845 6.876800209478922 7.191018591254256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.5 11 1.5 12 2.0 13 2.0 14 2.5 15 4.5 16 4.5 17 3.0 18 8.0 19 13.0 20 26.5 21 40.0 22 37.0 23 46.0 24 68.5 25 79.0 26 80.0 27 115.0 28 165.0 29 181.0 30 184.5 31 197.0 32 221.5 33 237.0 34 230.0 35 234.0 36 257.0 37 269.0 38 307.0 39 340.5 40 355.5 41 386.0 42 397.0 43 505.5 44 988.0 45 1459.0 46 1556.0 47 1037.5 48 474.0 49 366.5 50 304.0 51 301.5 52 299.0 53 276.5 54 254.0 55 240.5 56 251.5 57 230.0 58 184.0 59 186.0 60 177.5 61 160.0 62 153.0 63 133.5 64 101.0 65 82.5 66 77.5 67 78.0 68 64.0 69 56.0 70 53.5 71 45.0 72 42.0 73 44.5 74 42.5 75 35.0 76 42.5 77 40.0 78 32.5 79 35.0 80 32.0 81 40.0 82 51.5 83 52.0 84 51.0 85 57.0 86 66.5 87 69.0 88 75.5 89 88.5 90 108.5 91 137.0 92 152.0 93 164.5 94 192.0 95 265.0 96 323.0 97 406.5 98 762.0 99 8758.5 100 16483.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032731081434930615 2 0.009819324430479184 3 0.009819324430479184 4 0.0 5 0.0 6 0.0032731081434930615 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0016365540717465307 14-15 0.0016365540717465307 16-17 0.0032731081434930615 18-19 0.0016365540717465307 20-21 0.0016365540717465307 22-23 0.004909662215239592 24-25 0.006546216286986123 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.004909662215239592 36-37 0.004909662215239592 38-39 0.0016365540717465307 40-41 0.0016365540717465307 42-43 0.0 44-45 0.0032731081434930615 46-47 0.004909662215239592 48-49 0.0032731081434930615 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.006546216286986123 58-59 0.0016365540717465307 60-61 0.0 62-63 0.0 64-65 0.004909662215239592 66-67 0.0032731081434930615 68-69 0.004909662215239592 70-71 0.008182770358732654 72-73 0.0016365540717465307 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 30552.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.939251112856766 #Duplication Level Percentage of deduplicated Percentage of total 1 81.40815370196813 22.74482848913328 2 9.208059981255857 5.1453260015710915 3 4.522024367385192 3.7902592301649647 4 2.0735707591377697 2.3173605655930873 5 1.1129334582942831 1.554726368159204 6 0.4686035613870665 0.7855459544383346 7 0.22258669165885664 0.43532338308457713 8 0.14058106841611998 0.3142183817753339 9 0.11715089034676662 0.2945797329143755 >10 0.6677600749765698 3.318931657501964 >50 0.0 0.0 >100 0.035145267104029994 1.3387012306886619 >500 0.0 0.0 >1k 0.011715089034676664 5.243519245875883 >5k 0.0 0.0 >10k+ 0.011715089034676664 52.716679759099236 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16106 52.716679759099236 No Hit CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG 1602 5.243519245875883 No Hit TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT 183 0.5989787902592302 No Hit CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG 119 0.3894998690756743 No Hit ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC 107 0.3502225713537575 No Hit TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG 47 0.15383608274417387 No Hit CTTATACACATCTGACGCTGCCGACGAGCGATCAAGTGTAGATCTCGGTG 43 0.14074365017020163 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 42 0.13747054202670855 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 39 0.12765121759622938 No Hit CTTATACACATCTGACGCTGCCGACGAGCGAACTAGTGTAGATCTCGGTG 38 0.12437810945273632 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 36 0.1178318931657502 No Hit GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 34 0.11128567687876408 No Hit GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 33 0.108012568735271 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.009819324430479183 0.0 12 0.0 0.0 0.0 0.013092432573972244 0.0 13 0.0 0.0 0.0 0.013092432573972244 0.0 14 0.0 0.0 0.0 0.013092432573972244 0.0 15 0.0 0.0 0.0 0.013092432573972244 0.0 16 0.0 0.0 0.0 0.013092432573972244 0.0 17 0.0 0.0 0.0 0.013092432573972244 0.0 18 0.0 0.0 0.0 0.026184865147944488 0.0 19 0.0 0.0 0.0 0.05564283843938204 0.0 20 0.0 0.0 0.0 0.06546216286986122 0.0 21 0.0 0.0 0.0 0.06546216286986122 0.0 22 0.0 0.0 0.0 0.11455878502225714 0.0 23 0.0 0.0 0.0 0.1341974338832155 0.0 24 0.0 0.0 0.0 0.16692851531814612 0.0 25 0.0 0.0 0.0 0.18002094789211837 0.0 26 0.0 0.0 0.0 0.18984027232259754 0.0 27 0.0 0.0 0.0 0.20620581304006286 0.0 28 0.0 0.0 0.0 0.21602513747054203 0.0 29 0.0 0.0 0.0 0.22257135375752815 0.0 30 0.0 0.0 0.0 0.23893689447499344 0.0 31 0.0 0.0 0.0 0.25857554333595184 0.0 32 0.0 0.0 0.0 0.28148730034040326 0.0 33 0.0 0.0 0.0 0.31421838177533384 0.0 34 0.0 0.0 0.0 0.3698612202147159 0.0 35 0.0 0.0 0.0 0.4615082482325216 0.0 36 0.0 0.0 0.0 0.5826132495417649 0.0 37 0.0 0.0 0.0 0.7102644671379943 0.0 38 0.0 0.0 0.0 0.9753862267609322 0.0 39 0.0 0.0 0.0 1.2241424456664047 0.0 40 0.0 0.0 0.0 1.5285415030112595 0.0 41 0.0 0.0 0.0 1.7511128567687877 0.0 42 0.0 0.0 0.0 2.091516103692066 0.0 43 0.0 0.0 0.0 2.3369992144540457 0.0 44 0.0 0.0 0.0 2.6904948939512963 0.0 45 0.0 0.0 0.0 3.007986383870123 0.0 46 0.0 0.0 0.0 3.3647551715108666 0.0 47 0.0 0.0 0.0 3.587326525268395 0.0 48 0.0 0.0 0.0 3.8851793663262635 0.0 49 0.0 0.0 0.0 4.173212882953653 0.0 50 0.0 0.0 0.0 4.399057344854674 0.0 51 0.0 0.0 0.0 4.588897617177271 0.0 52 0.0 0.0 0.0 4.824561403508772 0.0 53 0.0 0.0 0.0 5.115868028279654 0.0 54 0.0 0.0 0.0 5.364624247185127 0.0 55 0.0 0.0 0.0 5.60356114166012 0.0 56 0.0 0.0 0.0 5.911233307148469 0.0 57 0.0 0.0 0.0 6.12725844461901 0.0 58 0.0 0.0 0.0 6.418565069389893 0.0 59 0.0 0.0 0.0 6.542943178842629 0.0 60 0.0 0.0 0.0 6.785153181461116 0.0 61 0.0 0.0 0.0 6.942262372348782 0.0 62 0.0 0.0 0.0 7.096098455092957 0.0 63 0.0 0.0 0.0 7.315396700706992 0.0 64 0.0 0.0 0.0 7.524875621890548 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTTAC 15 0.0022041753 70.0 46 AAGCTTA 15 0.0022041753 70.0 45 CGGTGGT 245 0.0 54.285717 46 AAAAAGG 150 0.0 53.666668 70 TGGTCGC 235 0.0 53.617016 49 GGTGGTC 235 0.0 53.617016 47 GTGGTCG 235 0.0 53.617016 48 GTCGCCG 240 0.0 52.5 51 GTATCAT 240 0.0 52.5 57 TCGCCGT 240 0.0 52.5 52 CGTATCA 240 0.0 52.5 56 CTCGGTG 240 0.0 52.5 44 GATTACT 20 0.0068877614 52.5 1 GGTCGCC 240 0.0 52.5 50 CCGTATC 245 0.0 51.428574 55 TCTCGGT 245 0.0 51.428574 43 TATCATT 245 0.0 51.428574 58 GCCGTAT 245 0.0 51.428574 54 CGCCGTA 245 0.0 51.428574 53 TCGGTGG 260 0.0 51.15385 45 >>END_MODULE