FastQCFastQC Report
Wed 25 May 2016
SRR1780843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30552
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT607419.880858863576854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28759.41018591254255No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT22507.364493322859388No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG8022.625032731081435No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT3391.1095836606441476RNA PCR Primer, Index 15 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC2750.9001047394605918No Hit
ATTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT2460.8051846032992929No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGACGT1890.6186174391201885No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATATCGTATGCCGT1360.4451427075150563No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCAGT1300.42550405865409796No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC1270.41568473422361873No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTAGCCAATCTCGTATGCCGT1220.3993191935061534No Hit
TATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG1180.3862267609321812No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGACAATCTCGTATGCCGT1090.35676878764074366No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGA1080.3534956794972506No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAGCCAATCTCGTATGCCGT1030.3371301387797853No Hit
ATTATAAACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT950.3109452736318408No Hit
CTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT910.2978528410578685No Hit
ATTATACACATCACCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT830.27166797590992403No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCACGTATGCCGT740.2422100026184865No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC710.23239067818800735No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAAACTCGTATGCCGT650.21275202932704898No Hit
ATTATACACATCTCCGAGCCAACGAGACGCTTGCCAATCTCGTATGCCGT570.18656716417910446No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGACAATCTCGTATGCCGT560.1832940560356114No Hit
CTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC550.18002094789211837No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCTATCTCGTATGCCGT520.17020162346163917No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGAATGCCGT510.16692851531814612No Hit
TATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCT490.16038229903116RNA PCR Primer, Index 15 (95% over 21bp)
TCTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCG470.15383608274417387No Hit
TTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC450.14728986645718775No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCAAATCTCGTATGCCGT430.14074365017020163No Hit
ATTATACACATCTCCGAGACCACGAGACGCTTGCCAATCTCGTATGCCGT410.1341974338832155No Hit
ATTATACACATCTCAGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT380.12437810945273632No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTAAGCCGT380.12437810945273632No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAAGCTCGTATGCCGT370.12110500130924325No Hit
ATTATACACATCTCCGAGCCCACGAGCCGCTTGCCAATCTCGTATGCCGT370.12110500130924325No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGCCAATCTCGTATGCCGT370.12110500130924325RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGA350.11455878502225714No Hit
ATTATACACAACTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT350.11455878502225714No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC350.11455878502225714No Hit
AATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT340.11128567687876408No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGCCAATCTCGTATGCCGT330.108012568735271No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC320.10473946059177797No Hit
ATTATACAAATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT310.10146635244828489No Hit
ATTATACACATCTCCGAGCACACGAGACGCTTGCCAATCTCGTATGCCGT310.10146635244828489No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAT455.456968E-1270.000011
CAGCTAG150.002204175370.054
CAAGAGA207.80593E-570.021
ACTCGTA401.2732926E-1070.038
CAGCATG150.002204175370.054
TCTAGTA207.80593E-570.038
CTTTATA150.002204175370.02
TGCCGTA150.002204175370.045
GCTAGAC207.80593E-570.029
ACGCATG550.070.027
ACGTCAT207.80593E-570.047
TATGCTT252.775314E-670.053
ACCACGA252.775314E-670.019
TAAGCCG252.775314E-670.043
ATCTAGT207.80593E-570.037
ATCTACG150.002204175370.010
CTGATTG207.80593E-570.054
CGCTAGA252.775314E-670.028
TCAGCTA207.80593E-570.053
GCTACAA150.002204175370.056
Produced by FastQC (version 0.11.5)