FastQCFastQC Report
Wed 25 May 2016
SRR1780842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233362
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2907912.460897661144488No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG18810.8060438289010208No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT2360.10113043254685852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA150.002220679570.0158653
ACCGGTT150.002224458869.98585551
GGTCGAC150.002224458869.98585550
CCGGTTG200.00693890652.51189852
CCGACTG200.006944791452.50064535
GACCTTC200.006944791452.50064539
GGTTATC200.006944791452.5006451
CTCGTTC200.00695068152.48939528
GTCGACG200.00695068152.48939551
ATGGCCG200.00695068152.48939524
TTGTACG200.00695068152.48939510
TATGCCC200.00695068152.48939551
TGCGCGT200.00695068152.4893955
GGACGCT200.00695068152.4893956
TGCTATT200.00695068152.48939524
TGGTCGA200.00695068152.48939549
TCGGTGG3500.051.98949445
GCCGTAT3450.050.7361354
AGATCTC3600.050.55617540
GATCTCG3600.050.55617541
Produced by FastQC (version 0.11.5)