FastQCFastQC Report
Wed 25 May 2016
SRR1780842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233362
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT113924.881685964295815No Hit
ATTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT58542.5085489497004656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34411.4745331287870347No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG22160.9495976208637226No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC9580.41052099313512913No Hit
ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT5220.22368680419262774TruSeq Adapter, Index 8 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC4750.20354642143965168No Hit
CTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT3320.14226823561676707No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC2500.10712969549455352No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACA150.00222092270.0139335
TTTCGAG252.8377654E-669.9839331
GAAGGTC150.002224701769.9839314
GCCGTAT453.8380676E-1062.20793546
CGTATGA1250.061.6122641
CGTCTAC401.0604708E-861.2621948
AGGGGGG19000.060.66934270
ATTATAC9900.057.637731
AGTCTCG252.3847283E-456.01114736
CCCGCTA252.389786E-455.98714414
TGCCGTA509.786163E-1055.98714445
TGACGTC1150.054.77003545
ATATCGA1100.054.0784930
ATATCGT1050.053.34395237
CAGCTTG1600.052.52171354
ATCGTAT1200.052.51045239
TGTCGGA200.00693966252.51045235
GGGCTTA200.00694554852.49926
AGACCTT200.00694554852.499225
CTCAGTC200.00694554852.49927