FastQCFastQC Report
Wed 25 May 2016
SRR1780841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47645
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2619754.98373386504355No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG16423.4463217546437193No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT2000.419771224682548No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1330.27914786441389444No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC1230.25815930317976704No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560.11753594291111344No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG520.10914051841746249No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG520.10914051841746249No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.10704166229404974No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCAAGTGTAGATCTCGGTG510.10704166229404974No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAAA150.002209273470.0199841
ACTGCCC150.002218515469.9464312
GCTGGCG150.002218515469.9464317
GCTAAAT200.006903532452.51498442
AAAGGGG305.8142305E-446.67998569
TGGTCGC2150.045.59440649
GTGGTCG2150.045.59440648
GTAGATC2400.045.22123738
TCGGTGG2400.045.22123745
GATCTCG2400.045.22123741
AGATCTC2400.045.22123740
TAGATCT2400.045.22123739
TCTCGGT2250.045.1239943
CGGTGGT2350.044.69360446
TGTAGAT2350.044.69360437
CTCGGTG2350.044.69360444
ATCTAGT2350.044.6466631
GGTCGCC2050.044.40291650
ATCTCGG2450.044.2983542
GTGTAGA2450.044.25182336