FastQCFastQC Report
Wed 25 May 2016
SRR1780841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47645
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT1090322.883828313569104No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT42298.876062545912477No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29626.216811837548536No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG20624.32784132647707No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC7231.5174729772274111No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT6131.2865988036520095No Hit
ATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTCTT4080.8563332983523979RNA PCR Primer, Index 40 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACCTGACCGAATCTCGTATGCCGT3190.6695351033686641No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC2900.6086682757896946No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT2270.47644034001469204No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC2080.43656207366984995No Hit
ATTATACACATCTCCGCGCCCACGAGACCTGACCGAATCTCGTATGCCGT1830.38409067058453145No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACAGAATCTCGTATGCCGT1630.34211354811627664No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGACGT1590.33371812362262565No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGACGT1430.30013642564802184No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGCATCTCGTATGCCGT1300.2728512960436562No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTAAGCCGT1120.23507188582222688No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCG1030.2161821807115122No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGAACGAATCTCGTATGCCGT1010.21198446846468674No Hit
CTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTC980.2056879000944485No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTAAGCCGT970.20358904397103578No Hit
TATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG940.19729247560079755No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATATCGTATGCCGT910.19099590723055934No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATATCGTATGCCGT890.18679819498373387No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGA850.1784027704900829No Hit
TCTTATACACATCTCCGCGCCCACGAGACCTGACCGAATCTCGTATGCCG850.1784027704900829No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCAGT740.15531535313254277No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGACCGAATCTCGTATGCCGT690.14482107251547907No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACAGAATCTCGTATGCCGT680.1427222163920663No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTAGTATGCCGT640.13432679189841537No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGA630.1322279357750026No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCAGT580.12173365515793892No Hit
ATTATACACATCTCCGAGCCCACGAGACCTGACCGCATCTCGTATGCCGT560.11753594291111344No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTGACCGAATCTCGTATGCCGT530.11123937454087522No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAAACTCGTATGCCGT490.10284395004722426No Hit
CTTATACACATCACCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT490.10284395004722426No Hit
CATATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT480.10074509392381152No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGGG150.00221192169.99895534
TTCCGTC150.00221192169.99895545
AACGAGC150.00221192169.99895513
GCGACCT150.00221192169.99895524
AACGAAT301.0034455E-769.99895532
ATGACCG252.7995993E-669.99895529
GTCGTCT150.00221192169.99895550
CACGCGA150.00221192169.99895521
TGCCGAC301.0034455E-769.99895545
CCACGCG150.00221192169.99895520
CTCTCGT252.7995993E-669.99895537
ACGTATT150.00221192169.99895547
GAGCCCC150.00221192169.99895516
CCGTCGT150.00221192169.99895548
CCCACGC150.00221192169.99895519
ACCGAGT150.00221192169.99895533
GACATGA252.7995993E-669.99895526
CCCCGAG150.00221192169.99895520
AAGACGT150.00221192169.99895544
AGACGTC150.00221192169.99895545
Produced by FastQC (version 0.11.5)