FastQCFastQC Report
Wed 25 May 2016
SRR1780840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255255
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243859.553191906133081No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG10550.4133121780180603No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGC252.3875028E-455.9999968
GCATAAG407.4612217E-752.51
TTGCCCG200.00694587452.529
AGGTGCT200.00694587452.563
CGAGAAT305.870764E-446.66666414
GTCGCCG1950.044.87179651
TCGCCGT1900.044.21052652
GTAGATC2200.042.95454438
CTCGGTG2200.042.95454444
TCGGTGG2300.042.60869645
ATCTCGG2200.041.36363642
CGGTGGT2200.041.36363646
GATCTCG2200.041.36363641
TGGTCGC2250.040.44444749
GCCGTAT2000.040.2554
TTAGGTG350.001253934740.049
AGGAGCG350.001253934740.011
TAGATCT2450.040.039
AGATCTC2450.038.5714340
TCTCGGT2450.038.5714343
Produced by FastQC (version 0.11.5)