FastQCFastQC Report
Wed 25 May 2016
SRR1780840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255255
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT113154.4328220798809035No Hit
ATTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT34671.358249593543711No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20700.8109537521302227No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG20650.8089949266419854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC8330.32634032634032634No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCCGTGCATCTCGTATGCCGT4270.1672836966954614No Hit
ATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGTCTT3810.14926250220367868RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA150.002222941569.999825
AATCTCG453.8380676E-1062.22204636
AGGGGGG20500.061.97543770
ATCGTAT352.96679E-759.99983239
GCCGTCA1950.059.23060646
TGCCGTA308.361647E-658.33316845
GACACCT252.3875356E-455.99984716
GCCGTAT252.3875356E-455.99984746
ATCTCGT26150.055.4109337
CGTATGC25750.055.1843141
TCTCGTA26350.055.12318438
CATCTCG26500.055.07531736
CGAATGC700.054.99984741
TATGCCG25300.054.7824543
TCGTATG26500.054.67909240
ATGCCGT25550.054.65738344
CTCGTAT26200.054.6372539
GTGCATC26850.054.61809533
TGCCGTC25450.054.4595745
GCATCTC26800.054.45880535