FastQCFastQC Report
Wed 25 May 2016
SRR1780839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283044
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229158.09591441613318No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG16780.592840689080143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA352.966135E-760.005151
CCGAATC308.3683535E-658.32803335
TCAGATG252.3869354E-456.0048074
GCCGTAT3250.054.92778854
GATCTCG3350.053.2787441
CGGTGGT3550.053.23459646
CTCGGTG3450.052.7488344
CGCGGCA200.00694429152.5045112
GCGGGTG200.006949146352.4952323
CGAATCC200.006949146352.4952336
TTCTGCG200.006949146352.4952332
TCGGTGG3600.052.49522845
TCGCCGT3450.051.7344352
ATCTCGG3500.050.9953642
AGATCTC3550.050.2771240
CGCCGTA3700.049.1934153
GTCGCCG3650.048.89966651
TGGTCGC3650.048.89966649
TCTCGGT3800.047.89038543
CGTATCA3750.047.60408456
Produced by FastQC (version 0.11.5)