FastQCFastQC Report
Wed 25 May 2016
SRR1780839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283044
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT87643.096338378485324No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT48291.7060951654159777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30581.080397394044742No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG17490.6179251282486116No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC8790.3105524229448425No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC4390.15509956049236162No Hit
ATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGTCTT4300.15191984285128815RNA PCR Primer, Index 45 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACGC150.00222107570.0166226
CACGAAT150.002222632570.0042439
GACCGTG150.00222419169.991877
GCCGTAT207.922866E-569.9918746
TGCCGTA301.0223266E-769.9918745
AGGGGGG16800.061.25370870
CGTATGA700.060.00363541
CGAATGC553.6379788E-1157.27620341
CGTCTTC21800.054.58082248
CGTCTTA452.696288E-854.43812648
CCGTCTT21900.054.1717947
GTATGCC23000.054.1676242
GCCGTCT22500.054.1270546
CTCGTAT24000.053.96160539
TATGCCG23200.053.85150543
TCGTATG24250.053.83831440
ATGCCGT23250.053.58517544
CGTATGC23800.053.23851841
TGCCGTC23100.053.1756445
ATCTCGT24000.053.0865537