FastQCFastQC Report
Wed 25 May 2016
SRR1780838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190681
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2829314.83787057965922No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG18860.9890864847572647No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT2310.12114473911926202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCG252.3883505E-455.9886744
GCCGTAT3200.053.61102754
CTTCGAG200.00694874852.4893831
CTCGTTA200.00694874852.4893818
CCTACCG200.00694874852.4893849
ACGGGTG200.00694874852.489388
GTGAATT200.00694874852.4893824
TGGTCGC3200.052.4893849
TCGCCGT3300.051.97281352
CGGTGGT3450.051.74223746
GTCGCCG3450.051.72866451
CGCCGTA3350.051.21053353
CTCGGTG3300.050.925544
ATCTCGG3450.050.74099342
TCGGTGG3450.050.7276845
GTATCAT3250.050.63170257
CGTATCA3400.050.45743656
GTAGATC3550.050.28470638
GATCTCG3500.050.0161241
TTGACGT352.0833586E-549.9898874
Produced by FastQC (version 0.11.5)