FastQCFastQC Report
Wed 25 May 2016
SRR1780838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190681
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT126526.6351655382549914No Hit
ATTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT54832.875483136757202No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33691.7668252211809252No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG23261.2198383687939542No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC9340.48982331747788194No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT6790.3560921119566186RNA PCR Primer, Index 15 (95% over 23bp)
ATCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC3800.1992857180316864No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC3590.18827255992993536No Hit
CTTATACACATCTCCGCGCCCACGAGACTAATGTCTATCTCGTATGCCGT3100.16257519102584947No Hit
ATTATACACATCTCCGCGCCCACGAGACTAATGTCTATCTCGTATGCCGT1970.10331391171642691No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGAC150.002222744369.9944945
AGGGGGG22450.063.93155770
ATTATAC10300.060.1566771
AGCCGTC352.9643343E-759.99527745
GCCGTCA1050.056.66220546
TCTGCCG252.3868914E-455.99559443
TGTCAAT700.054.9956732
CGTATGA1150.054.77829441
CGTCTTC30900.053.81246648
GCCGTCT30400.053.7622246
CCGTCTT30150.053.51154347
CTTGAAA31700.053.4342857
TTGAAAA32350.053.33429358
GTCTTCT30700.053.25081649
TGCCGTC31450.053.1913645
ATGCCGT31550.053.0227744
CTGCTTG30400.052.9702554
TATGCCG31150.052.91718743
TCTTCTG31000.052.84841550
GTATGCC31300.052.77540242