FastQCFastQC Report
Wed 25 May 2016
SRR1780837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215679
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3304915.323234992743846No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG19440.9013394906319113No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT2480.11498569633575824No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC2240.1038580483032655No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTCG150.002222534369.99930641
TGCGCGT150.002222534369.9993065
CACTAGT150.002224579769.9830830
CTCTCGG150.002224579769.9830842
TCGCCAG200.00693832152.51165848
GCATTCA200.00693832152.5116581
CTCTGGA200.00693832152.5116581
CGTGCGA200.006944689452.4994810
CCCGTGC200.006944689452.499488
CGCCGTG200.006944689452.4994853
TGTACGC200.006944689452.4994868
TCCTTCC200.006944689452.4994818
CCGTATA200.00695106352.4873123
AGTCTTA200.00695106352.4873127
ACCCGTA200.00695106352.4873121
TCGCCGT3700.050.13463652
GCCGTAT3600.047.63841654
CGGTGGT3950.046.9724546
GTCGCCG4100.046.96164351
TAGAGCG305.8623013E-446.6770334
Produced by FastQC (version 0.11.5)