FastQCFastQC Report
Wed 25 May 2016
SRR1780837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215679
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT137096.35620528656012No Hit
ATTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT52432.4309274430983083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37441.7359130930688662No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG25671.1901946874753684No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC10760.4988895534567575No Hit
ATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGTCTT6580.30508301689084244RNA PCR Primer, Index 31 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC3860.17896967252259144No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC3740.17340584850634508No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGAGATATCTCGTATGCCGT3660.16969663249551417No Hit
CTTATACACATCTCCGCGCCCACGAGACCCCGAGATATCTCGTATGCCGT3350.1553234204535444No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC207.900403E-570.0243666
TAACTCG252.8273134E-670.0243636
AGATAAC252.8312134E-670.00812533
CTGCCGT252.8312134E-670.00812544
GCCGACA301.0195254E-770.0081246
TCTGCCG252.8390277E-669.97565543
CGTCTTA900.062.24387448
AGGGGGG25600.061.92641470
TGCCGAC750.060.67370245
CGAATGC650.059.223941
ATTATAC10400.059.22391
TGACGTC1200.058.34009645
CGTCTGC308.362864E-658.3265741
TCGAATG601.8189894E-1258.3265740
GTCTGCC308.374371E-658.31304642
CGTCTAC553.45608E-1157.2926648
CTCGTTT252.3825651E-456.0194939
AATCTCG700.055.0191436
AACTCGT452.6824637E-854.4633937
ACGAGAT1250.053.18149630
Produced by FastQC (version 0.11.5)