FastQCFastQC Report
Wed 25 May 2016
SRR1780836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169436
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2725616.086309875115088No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG16820.9927052102268703No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT2190.12925234306758893No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTC150.002219539670.0165460
GCTGCGG150.002222141569.99586517
CTGCGGG150.002222141569.99586518
CGTGTGC150.002224745769.9752041
GCGTCTA150.002224745769.9752041
GCGCGTC252.3858961E-455.9966936
TCGTTCC200.006935372552.512462
CGTTTCG200.006935372552.512461
CGGCGAG200.00694347952.496922
GTCTGGC407.449198E-752.496910
TGCGGGC200.00694347952.496919
TCAGATG200.00695159352.4814034
TGGCGCT506.162372E-848.99710513
TCTTTGA305.8583054E-446.67769248
CGCGTCT305.866843E-446.663917
TGCCGGC305.866843E-446.6639119
GGCGCTG757.4942363E-1041.99752414
TCTCGGT3700.040.68528743
ATCTCGG3650.040.28348542
CGGTGGT3750.040.1428246
Produced by FastQC (version 0.11.5)