FastQCFastQC Report
Wed 25 May 2016
SRR1780836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169436
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT142078.384876885667744RNA PCR Primer, Index 14 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT31331.8490757572180647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30511.8006799027361364No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG24041.4188248070067755No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC11550.68167331617838RNA PCR Primer, Index 14 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCTT5160.30453976722774384RNA PCR Primer, Index 14 (96% over 26bp)
CTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT4980.29391628697561323RNA PCR Primer, Index 14 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC3250.19181283788569137RNA PCR Primer, Index 14 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC2250.1327935031516325RNA PCR Primer, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGC150.00222102770.004726
ATAACGA150.00222102770.0047237
CGTATGA750.065.33774641
AGGGGGG21300.061.47769570
CCGACTT401.0593794E-861.2541347
TGACGTC700.060.0040545
CGTCTTA650.059.21727848
GCATATC553.6379788E-1157.2596835
GTATGAC800.056.87883842
CGAATGC750.056.0037841
TACATGC252.384405E-456.0037772
AATCTCG252.384405E-456.00377736
ATTATAC6200.054.761761
ATCGTAT452.683737E-854.4481239
CGACTTC452.6901034E-854.43204548
TTAATAC200.006940004452.5035442
GGGCTCG200.006940004452.5035441
GGCCCCC200.006940004452.5035441
GATCTTA200.006940004452.5035441
AGCTAGA407.4427044E-752.50354455
Produced by FastQC (version 0.11.5)