FastQCFastQC Report
Wed 25 May 2016
SRR1780835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155752
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2894518.58403102367867No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG19711.2654733165545227No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT2520.1617956751759207No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC2000.12840926601263547No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1870.12006266372181418No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAA150.00221958770.0134968
GGACCTA150.00221958770.013491
ACACGTC150.002222416569.991016
TCTGGCG352.961806E-759.992311
CGGTGGT3050.052.79705446
CGCTGCG200.006935525752.51011716
TTGCGTG200.006935525752.51011732
CGTCCCA200.006935525752.51011714
TCGGTGG3000.052.51011745
TCGCCGT3000.052.4932652
CGTGCGA200.00694434152.4932610
GGCACGC200.00694434152.4932652
GCTCTAT200.00694434152.493263
ATTCAAT200.00694434152.4932626
TGGTCGC3100.051.94548849
GCCGTAT3000.051.32674454
CGTATCA3150.049.9935856
GTCGCCG3250.049.54800851
CTCGGTG3050.049.33792544
GTGGTCG3300.048.7972848
Produced by FastQC (version 0.11.5)