##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780835_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 155752 Sequences flagged as poor quality 0 Sequence length 76 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.004905233961683 32.0 27.0 32.0 13.0 32.0 2 29.105931223997125 32.0 32.0 32.0 13.0 32.0 3 28.919744208742102 32.0 32.0 32.0 13.0 32.0 4 28.197153166572498 32.0 27.0 32.0 13.0 32.0 5 28.88995325902717 32.0 32.0 32.0 13.0 32.0 6 32.74249447840156 37.0 32.0 37.0 13.0 37.0 7 32.52485361343675 37.0 32.0 37.0 13.0 37.0 8 32.700523909805334 37.0 32.0 37.0 13.0 37.0 9 32.69392367353228 37.0 32.0 37.0 13.0 37.0 10-11 32.519855282757206 37.0 32.0 37.0 13.0 37.0 12-13 32.38345575016693 37.0 32.0 37.0 13.0 37.0 14-15 33.04400906569418 37.0 34.5 37.0 17.5 37.0 16-17 33.09364245723972 37.0 34.5 37.0 17.5 37.0 18-19 32.54095613539473 37.0 32.0 37.0 13.0 37.0 20-21 32.99506266372181 37.0 34.5 37.0 17.5 37.0 22-23 32.888669166367045 37.0 34.5 37.0 17.5 37.0 24-25 32.37824554419847 37.0 32.0 37.0 13.0 37.0 26-27 32.82259296830859 37.0 34.5 37.0 17.5 37.0 28-29 32.69060429400585 37.0 32.0 37.0 13.0 37.0 30-31 32.58395397811906 37.0 32.0 37.0 13.0 37.0 32-33 32.77404463506086 37.0 32.0 37.0 13.0 37.0 34-35 32.56267656274077 37.0 32.0 37.0 13.0 37.0 36-37 33.12633224613488 37.0 34.5 37.0 17.5 37.0 38-39 33.26486016230931 37.0 34.5 37.0 17.5 37.0 40-41 32.744054650983614 37.0 32.0 37.0 17.5 37.0 42-43 32.57024307874056 37.0 32.0 37.0 13.0 37.0 44-45 32.30232677590015 37.0 32.0 37.0 13.0 37.0 46-47 32.55612447994247 37.0 32.0 37.0 13.0 37.0 48-49 33.0609109353331 37.0 34.5 37.0 17.5 37.0 50-51 33.33398929066722 37.0 34.5 37.0 22.0 37.0 52-53 32.39179593199445 37.0 32.0 37.0 13.0 37.0 54-55 32.5076756638759 37.0 32.0 37.0 13.0 37.0 56-57 32.27056153372027 37.0 32.0 37.0 13.0 37.0 58-59 31.916280368791412 37.0 32.0 37.0 13.0 37.0 60-61 31.705917098977864 37.0 32.0 37.0 13.0 37.0 62-63 31.426983281113564 37.0 29.5 37.0 13.0 37.0 64-65 31.384893933946273 37.0 29.5 37.0 13.0 37.0 66-67 31.390637038368688 37.0 29.5 37.0 13.0 37.0 68-69 31.539601417638295 37.0 32.0 37.0 13.0 37.0 70-71 31.6016487749756 37.0 32.0 37.0 13.0 37.0 72-73 31.856152729980995 37.0 32.0 37.0 13.0 37.0 74-75 32.01444604242642 37.0 32.0 37.0 13.0 37.0 76 31.6839334326365 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.8256516687526094 11101 2 -0.19961569620570074 11101 3 -0.45923790164766487 11101 4 -0.42207285296344565 11101 5 0.09455182177773835 11101 6 -2.098392684998302 11101 7 -0.3772073723381766 11101 8 -0.8856007384006226 11101 9 -1.0712992209665018 11101 10-11 -0.14050924017128352 11101 12-13 0.034377590099772704 11101 14-15 -1.1256481202019302 11101 16-17 -0.029939544208026803 11101 18-19 -0.9416320370856965 11101 20-21 -0.6041553383856169 11101 22-23 -0.80449284249854 11101 24-25 -0.7522604815444112 11101 26-27 -1.9154690896663595 11101 28-29 -1.068937633381264 11101 30-31 -0.3614347947885008 11101 32-33 -0.8631636097963629 11101 34-35 -1.0249171686066738 11101 36-37 -0.48640701828533395 11101 38-39 -0.4771389081458679 11101 40-41 -0.7403230538163257 11101 42-43 -1.927650337554013 11101 44-45 -0.7679231787039669 11101 46-47 -2.0814684618501893 11101 48-49 -1.1052976783455435 11101 50-51 -0.6302256656916043 11101 52-53 -0.9876216878006119 11101 54-55 -1.4268140605906439 11101 56-57 -1.96155994083243 11101 58-59 -1.2367422832165538 11101 60-61 -0.719988387326822 11101 62-63 -2.2404050579095127 11101 64-65 -0.2751992422366598 11101 66-67 -0.8657168604445893 11101 68-69 -0.9557672414996006 11101 70-71 -1.8110072067823388 11101 72-73 -1.335900909336182 11101 74-75 -1.3224084405186076 11101 76 -0.825283824769361 11102 1 0.6497334812531825 11102 2 -0.09281620587788808 11102 3 -1.6575467166598266 11102 4 0.011727679875658481 11102 5 -0.3159659588315513 11102 6 -1.0339886539546335 11102 7 -1.3724581542216505 11102 8 0.5228320196209211 11102 9 -0.6911301024677172 11102 10-11 -1.1860901506311485 11102 12-13 -0.5205859220049405 11102 14-15 -1.8093617770998804 11102 16-17 -1.459570031871646 11102 18-19 -0.42141890144339555 11102 20-21 -0.22653458082741196 11102 22-23 -0.0340190790327668 11102 24-25 -1.3008530912976823 11102 26-27 -1.1287205665573623 11102 28-29 -0.5501145127974567 11102 30-31 0.10839029498330888 11102 32-33 -0.9283784829574913 11102 34-35 -1.6743437873950455 11102 36-37 -0.9824686422862641 11102 38-39 -1.7013367536225275 11102 40-41 -1.73609509134673 11102 42-43 -0.8239783811078176 11102 44-45 -2.007816610882813 11102 46-47 -1.4948241898705739 11102 48-49 -0.30586526782776247 11102 50-51 -1.2827798183615897 11102 52-53 -0.848041009009922 11102 54-55 -0.7233506064032262 11102 56-57 -1.1604479287856293 11102 58-59 -0.997659693155164 11102 60-61 -1.029845910783326 11102 62-63 -1.0153384530212932 11102 64-65 -0.525344035471921 11102 66-67 -0.9902969600623379 11102 68-69 -0.6899268615621494 11102 70-71 -0.8356220567765469 11102 72-73 -1.0383218522297284 11102 74-75 -0.47502051048270033 11102 76 -1.2311682218503286 11103 1 0.4383932750676145 11103 2 -0.6146718759809815 11103 3 -0.05373228367013638 11103 4 -0.2572413922892878 11103 5 0.5870237318900955 11103 6 -0.6874938085938069 11103 7 1.0453768973247435 11103 8 -0.573353547389388 11103 9 0.4382513408312505 11103 10-11 0.10932222050287521 11103 12-13 0.8704450058301063 11103 14-15 -1.0931762100895668 11103 16-17 0.2657907928706251 11103 18-19 0.3809522325772221 11103 20-21 -5.036529923572175E-4 11103 22-23 -0.6749984604760684 11103 24-25 0.3210541251971648 11103 26-27 -0.2561432469697351 11103 28-29 0.09179270369739356 11103 30-31 0.29019441869464657 11103 32-33 0.7411060531249802 11103 34-35 0.6532850785843323 11103 36-37 -0.18865420929657262 11103 38-39 0.22196221544964345 11103 40-41 0.24344099112749973 11103 42-43 0.7343721343561 11103 44-45 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-0.1795683529491825 11104 24-25 -0.016849100609285017 11104 26-27 2.0083905177159522E-4 11104 28-29 1.0618464671382526 11104 30-31 -0.5680313877569674 11104 32-33 -0.6068509361223278 11104 34-35 0.6552205624553054 11104 36-37 -0.20478324155463667 11104 38-39 0.5269084520087759 11104 40-41 -0.9182256755391691 11104 42-43 -0.018746145213796694 11104 44-45 0.4725625045145918 11104 46-47 -1.2352077393661958 11104 48-49 0.6099977185373788 11104 50-51 -0.9302135840195049 11104 52-53 -1.119130688646326 11104 54-55 -0.3126422592133338 11104 56-57 0.4132135278156355 11104 58-59 -0.23571534108776504 11104 60-61 -0.5374947302046778 11104 62-63 -0.5907735489086647 11104 64-65 -0.4567263655905336 11104 66-67 -2.0946645468043847 11104 68-69 -0.6988504842203938 11104 70-71 -0.4609407575857709 11104 72-73 -0.3204967774043226 11104 74-75 0.31036913589797166 11104 76 -2.3272773006664274 11105 1 0.35774811377729065 11105 2 -0.21918800501324043 11105 3 0.7921816948244889 11105 4 1.465446779753723 11105 5 1.1277764200621405 11105 6 0.2362696322664064 11105 7 0.5661295854967818 11105 8 0.026323871965452383 11105 9 -0.4644368312117564 11105 10-11 -0.30137670422831064 11105 12-13 0.3739933929268773 11105 14-15 -0.22683212406806774 11105 16-17 0.18148971760180643 11105 18-19 0.14148986698582178 11105 20-21 -0.21367569600311498 11105 22-23 -0.05591243897068665 11105 24-25 1.1121831574552274 11105 26-27 -0.18797120395898048 11105 28-29 0.21776044563286945 11105 30-31 0.001861085361319681 11105 32-33 -0.30039932321910356 11105 34-35 -0.45768266335115015 11105 36-37 0.35973288747761956 11105 38-39 -0.05373670928154439 11105 40-41 0.544139915858679 11105 42-43 0.029640951560402584 11105 44-45 0.05858400989093582 11105 46-47 0.8185557014940201 11105 48-49 -0.545916259957238 11105 50-51 -0.005482401223801503 11105 52-53 0.35936393500958985 11105 54-55 0.47768032143182637 11105 56-57 -0.4900122786359802 11105 58-59 0.32342444385846747 11105 60-61 2.037774086999626 11105 62-63 -0.5800208607366244 11105 64-65 0.5432736344094664 11105 66-67 0.1418945929805595 11105 68-69 0.8871710211559503 11105 70-71 0.3777689198335814 11105 72-73 1.7010085989397652 11105 74-75 -0.6681254877579441 11105 76 0.3716474305163686 11106 1 1.2318715359371808 11106 2 1.2583716022798903 11106 3 0.2775720641559509 11106 4 1.5497151294498401 11106 5 0.8996324275422722 11106 6 1.769462713145323 11106 7 1.468420375585609 11106 8 1.8166464526106125 11106 9 -0.429139963516576 11106 10-11 0.37475700311156857 11106 12-13 1.234792831917062 11106 14-15 0.6576933551278188 11106 16-17 0.7064429667836691 11106 18-19 0.1376913630120029 11106 20-21 0.3766702600511209 11106 22-23 0.485871104741328 11106 24-25 0.7590976034580308 11106 26-27 -0.4087519426219046 11106 28-29 1.0913579210886937 11106 30-31 1.1232378969555228 11106 32-33 0.12871474877715627 11106 34-35 0.27198073075824425 11106 36-37 0.9961283993990833 11106 38-39 0.39957091110181153 11106 40-41 0.2756149041709719 11106 42-43 1.1826330039213175 11106 44-45 0.11141243438836312 11106 46-47 0.8277889880765343 11106 48-49 1.1285811687477647 11106 50-51 0.25918566796740805 11106 52-53 0.44392447731907936 11106 54-55 0.6842020605622636 11106 56-57 0.45844494436542504 11106 58-59 -0.34330066133086845 11106 60-61 0.9197282711978474 11106 62-63 -0.11473334320506723 11106 64-65 0.22168644413363836 11106 66-67 0.08298856212502059 11106 68-69 -0.2650613304101981 11106 70-71 0.10521165008136535 11106 72-73 0.9787668972099866 11106 74-75 -0.31685152796364946 11106 76 -0.8335886611152326 11107 1 -1.1037119880902786 11107 2 -0.059935033875717636 11107 3 -0.6111007047227659 11107 4 -0.6030308659734978 11107 5 -0.9987657417941129 11107 6 0.7404009282482988 11107 7 -0.46567573425420505 11107 8 -1.7359851263367574 11107 9 -0.8906960275898008 11107 10-11 0.010111694187081355 11107 12-13 -0.28271288890672963 11107 14-15 -1.092649894300095 11107 16-17 -0.07210394397148434 11107 18-19 0.2909522325772187 11107 20-21 0.11660161016553161 11107 22-23 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64-65 0.3449814142956136 11108 66-67 1.4681121767882779 11108 68-69 1.4758806985753026 11108 70-71 0.8157828350770977 11108 72-73 0.5639118247462207 11108 74-75 0.6960427601863941 11108 76 0.16513256650624797 11109 1 1.172435828259104 11109 2 0.06873237933816867 11109 3 -0.6935195177126872 11109 4 0.15956711834900972 11109 5 0.6383003276347772 11109 6 -0.0621746596576358 11109 7 -0.9127082090582377 11109 8 0.07196560154678622 11109 9 0.9584641067886963 11109 10-11 -0.5588692688588281 11109 12-13 0.175125856893942 11109 14-15 -0.19360174200446068 11109 16-17 -0.31101771776767606 11109 18-19 0.10797350917296455 11109 20-21 0.11811336828423435 11109 22-23 -0.24042399239095147 11109 24-25 -0.7443714067177325 11109 26-27 -0.21656877888462134 11109 28-29 -0.23831367928133318 11109 30-31 0.9681731420989053 11109 32-33 -0.614000329853738 11109 34-35 -0.4218213043943848 11109 36-37 -0.3535478263178504 11109 38-39 0.0664303005560214 11109 40-41 -0.3792185833405881 11109 42-43 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-0.6937478368431513 21610 30-31 -0.20985062635039498 21610 32-33 0.10849344051237608 21610 34-35 0.11148327678253622 21610 36-37 0.6311656105232473 21610 38-39 -0.08236210887468332 21610 40-41 -0.26507252238602064 21610 42-43 -0.27085309086912446 21610 44-45 -0.11694635918782126 21610 46-47 0.9420663088755887 21610 48-49 0.44536534422758933 21610 50-51 1.2666768548064198 21610 52-53 0.6023165650589917 21610 54-55 0.626328970080472 21610 56-57 1.8915628448746276 21610 58-59 0.6396115987553053 21610 60-61 0.41600715878108474 21610 62-63 0.631545544378163 21610 64-65 0.21488072537139757 21610 66-67 1.205393575834389 21610 68-69 -0.8777229050282962 21610 70-71 0.3899746739748231 21610 72-73 0.27787578871018326 21610 74-75 0.6227783192749001 21610 76 0.9020165092725421 21611 1 1.0550599417342816 21611 2 -0.29633854264764636 21611 3 0.20293438299653133 21611 4 -0.9705747256226225 21611 5 -0.05797626810990408 21611 6 -0.025827141927145192 21611 7 -0.3862897693419285 21611 8 1.1916464526106125 21611 9 -0.1034153258354138 21611 10-11 -0.05151111283046106 21611 12-13 0.6471116724967771 21611 14-15 1.1051571232437567 21611 16-17 -0.10587587379604457 21611 18-19 0.8326188992438901 21611 20-21 0.06616301367430566 21611 22-23 1.5500015395239402 21611 24-25 0.2585541251971648 21611 26-27 0.7571900863636003 21611 28-29 1.2009593703640604 21611 30-31 1.1685277520279769 21611 32-33 0.23777271979164993 21611 34-35 0.45911841191767166 21611 36-37 0.19051245737009026 21611 38-39 0.6586288821163038 21611 40-41 0.8359409911275009 21611 42-43 0.31687213435609607 21611 44-45 0.5442022894608272 21611 46-47 0.7774266692359575 21611 48-49 0.42532029918254466 21611 50-51 0.4908885665181302 21611 52-53 0.28396070920313576 21611 54-55 0.6526803214318235 21611 56-57 0.03525653856831923 21611 58-59 0.903800787944494 21611 60-61 0.4229891407630646 21611 62-63 0.8760275263601542 21611 64-65 1.1001284731191419 21611 66-67 0.9112494316902406 21611 68-69 0.8550473652419726 21611 70-71 0.5030377370378822 21611 72-73 -0.14025484192045212 21611 74-75 1.1480035445001207 21611 76 -0.1549654727094385 21612 1 0.4470139647227853 21612 2 -0.27536153115339346 21612 3 -0.1482150422908255 21612 4 -1.3648275991858405 21612 5 -0.33987281983404216 21612 6 0.29457515692342895 21612 7 -0.013588619916639288 21612 8 0.6959567974381997 21612 9 0.5296306511760775 21612 10-11 -0.07464329673850756 21612 12-13 -0.9919687872733398 21612 14-15 -0.09024517560681034 21612 16-17 -0.36420920712937743 21612 18-19 1.0383660256806664 21612 20-21 0.11673772631798585 21612 22-23 -0.7663777708208883 21612 24-25 -0.3694631161821462 21612 26-27 0.6497188219957835 21612 28-29 -0.3083797100957142 21612 30-31 -0.5656676502708677 21612 32-33 -0.31768705032329336 21612 34-35 0.06845749237744059 21612 36-37 -0.11537834722760465 21612 38-39 -0.870106750067599 21612 40-41 1.317406508368876 21612 42-43 -0.7568347621956235 21612 44-45 1.1164724044033534 21612 46-47 0.07685195659227162 21612 48-49 0.3960099543549589 21612 50-51 0.7614345435296244 21612 52-53 -0.2865852678083556 21612 54-55 0.5768182524663104 21612 56-57 0.0924404466142974 21612 58-59 -0.7085555338945859 21612 60-61 0.7255753476596212 21612 62-63 -0.5675069563984678 21612 64-65 0.11205376047546167 21612 66-67 -0.2146126372752768 21612 68-69 -0.18503884165457762 21612 70-71 0.7633825646240915 21612 72-73 -0.7967203591618315 21612 74-75 0.21725641806334295 21612 76 -0.056976966962309916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 8.0 12 36.0 13 114.0 14 163.0 15 286.0 16 444.0 17 651.0 18 844.0 19 1042.0 20 1398.0 21 1788.0 22 2203.0 23 2588.0 24 3141.0 25 3505.0 26 3830.0 27 4309.0 28 5015.0 29 5678.0 30 6937.0 31 8321.0 32 11092.0 33 16142.0 34 24415.0 35 31883.0 36 19917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.140378700808384 13.824056297875345 6.45422266169266 20.58134233962361 2 40.95552402293463 18.754775372880378 22.44666028880171 17.84304031538328 3 36.89864639252048 18.53504738910436 25.080266098168646 19.486040120206507 4 33.46666495454312 17.187580255791257 26.230160768401046 23.115594021264574 5 32.6469813997984 24.318944982696742 28.399175607219213 14.63489801028565 6 42.46494427037855 28.840079100107864 16.25661307719965 12.438363552313934 7 43.37128143881751 24.30476630773281 15.963246200373701 16.36070605307598 8 41.42434301343829 27.03837584832005 15.950664209722055 15.586616928519605 9 39.94182948205125 17.49545743471868 15.346486975839332 27.21622610739074 10-11 40.65496617944956 20.971309515019406 21.055098443965754 17.31862586156528 12-13 40.36957017242542 20.025232987797636 20.46472357679207 19.140473262984877 14-15 41.06814942277811 19.981572560450992 18.407535346012097 20.5427426707588 16-17 40.31988288510991 22.397259614304197 18.539980545059375 18.74287695552652 18-19 38.50248323375786 22.303195297426235 20.848569291371444 18.345752177444467 20-21 40.04224773351825 21.7717081439248 20.137337237075275 18.048706885481675 22-23 40.61895054495257 21.66455320310117 19.436909099664522 18.27958715228174 24-25 39.26939906323335 22.90134415419731 19.558133786200187 18.271122996369147 26-27 40.05072231139647 22.44558587479936 20.08378812199037 17.419903691813804 28-29 40.107607655809595 22.097450417653818 20.33662705215376 17.45831487438283 30-31 40.21245381264386 22.421292892845337 20.16866611236489 17.197587182145917 32-33 38.95100786840407 22.313394264508073 20.871656088783592 17.863941778304266 34-35 39.491730018491886 22.199250051366345 21.238057324840764 17.070962605301006 36-37 40.708422022531074 21.26710778506554 21.31430167490152 16.71016851750187 38-39 40.071783775454186 21.37461435482887 21.938996632326454 16.614605237390485 40-41 39.99268038986555 21.25479948120658 21.327995582551075 17.424524546376794 42-43 38.792760285582204 21.363770609687194 22.242732035543685 17.600737069186913 44-45 38.27934137407423 20.356215308654658 22.53890085618802 18.825542461083092 46-47 39.10713884421158 19.897397373295707 21.98668990956458 19.008773872928124 48-49 38.916123315772744 19.797876677806563 23.53471827617314 17.751281730247552 50-51 39.53533167892491 20.11454584562636 22.35441794465972 17.995704530789013 52-53 37.38896219176675 20.720208800839828 22.947546172787188 18.943282834606233 54-55 37.58740024012995 21.17316965116951 21.72341395450372 19.516016154196816 56-57 37.64161634450854 21.080088477108635 21.557466780954947 19.720828397427873 58-59 37.04047363723343 21.76541600580488 22.43355529727543 18.760555059686254 60-61 36.90381028510525 21.038269785777803 22.428964458954926 19.628955470162023 62-63 36.89502407704655 21.28860353130016 22.407383627608347 19.408988764044942 64-65 37.65799316178949 21.069393389729843 22.03252163025507 19.240091818225597 66-67 37.42587735209983 22.216407632024193 20.164570738770436 20.19314427710553 68-69 37.69631322794807 21.915490606500327 20.035506529862726 20.35268963568888 70-71 38.705141294088186 21.80539235515831 19.634131463538356 19.855334887215147 72-73 39.23414255170244 21.69287369901186 18.706941385708873 20.366042363576828 74-75 40.17136271560788 20.88390802014748 19.00687954851157 19.937849715733076 76 41.55773280599928 19.089963531768454 19.400071909188966 19.9522317530433 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 3.0 4 5.0 5 4.5 6 3.0 7 2.0 8 2.0 9 2.5 10 7.0 11 9.5 12 8.0 13 8.5 14 12.5 15 20.0 16 32.5 17 41.0 18 52.0 19 79.0 20 129.0 21 163.0 22 177.0 23 216.5 24 317.0 25 392.0 26 503.0 27 665.0 28 815.5 29 915.0 30 1073.5 31 1401.0 32 1884.5 33 2199.0 34 2351.5 35 2928.0 36 3681.0 37 4010.0 38 4259.0 39 5074.0 40 6162.5 41 6960.0 42 7235.0 43 7528.0 44 8573.5 45 8923.5 46 8521.0 47 8219.0 48 7679.5 49 6912.5 50 6383.0 51 5933.5 52 4933.0 53 4050.0 54 3718.0 55 3487.5 56 2885.5 57 2257.5 58 2001.0 59 1755.5 60 1345.0 61 1128.0 62 1076.0 63 928.5 64 730.0 65 624.5 66 542.0 67 514.0 68 512.0 69 480.0 70 418.5 71 387.0 72 352.5 73 306.0 74 300.0 75 306.0 76 278.5 77 244.0 78 232.5 79 228.0 80 214.0 81 199.5 82 175.5 83 152.0 84 152.5 85 161.5 86 171.0 87 172.0 88 173.5 89 197.0 90 211.5 91 246.0 92 288.0 93 288.0 94 363.0 95 499.5 96 561.0 97 728.0 98 1350.0 99 15564.5 100 29324.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005778416970568596 2 0.0032102316503158867 3 0.012840926601263547 4 0.0 5 6.420463300631774E-4 6 0.0 7 0.008346602290821306 8 0.001926138990189532 9 0.001926138990189532 10-11 0.0022471621552211207 12-13 0.0022471621552211207 14-15 0.003852277980379064 16-17 0.00481534747547383 18-19 0.00481534747547383 20-21 0.0025681853202527095 22-23 0.002889208485284298 24-25 0.0022471621552211207 26-27 0.0012840926601263548 28-29 6.420463300631774E-4 30-31 3.210231650315887E-4 32-33 0.0016051158251579433 34-35 0.005136370640505419 36-37 0.007383532795726539 38-39 0.004173301145410653 40-41 0.003852277980379064 42-43 0.0 44-45 0.0022471621552211207 46-47 0.0035312548153474755 48-49 0.0035312548153474755 50-51 0.004173301145410653 52-53 0.0035312548153474755 54-55 0.001926138990189532 56-57 0.0035312548153474755 58-59 0.013482972931326723 60-61 0.0016051158251579433 62-63 0.0012840926601263548 64-65 0.006099440135600185 66-67 0.008667625455852894 68-69 0.003852277980379064 70-71 0.008346602290821306 72-73 0.0032102316503158867 74-75 3.210231650315887E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 155752.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.09735990549078 #Duplication Level Percentage of deduplicated Percentage of total 1 73.15307262569833 31.52704299142226 2 8.822346368715085 7.604396733268273 3 6.106517690875233 7.895243720786891 4 4.227932960893855 7.28850993887719 5 2.7098696461824954 5.839411371924598 6 1.6089385474860336 4.160460218809389 7 0.8759776536312849 2.642662694540038 8 0.6122905027932961 2.111048333247727 9 0.43500931098696466 1.6872977554060302 >10 1.433147113594041 8.744671015460476 >50 0.0059590316573556795 0.1444604242642149 >100 0.0059590316573556795 0.5052904617597206 >500 0.0 0.0 >1k 0.0014897579143389199 1.2654733165545227 >5k 0.0 0.0 >10k+ 0.0014897579143389199 18.58403102367867 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 28945 18.58403102367867 No Hit CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG 1971 1.2654733165545227 No Hit TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT 252 0.1617956751759207 No Hit ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC 200 0.12840926601263547 No Hit CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG 187 0.12006266372181418 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0012840926601263548 0.0 2 0.0 0.0 0.0 0.004494324310442241 0.0 3 0.0 0.0 0.0 0.005136370640505419 0.0 4 0.0 0.0 0.0 0.008988648620884483 0.0 5 0.0 0.0 0.0 0.015409111921516257 0.0 6 0.0 0.0 0.0 0.017977297241768966 0.0 7 0.0 0.0 0.0 0.019903436231958498 0.0 8 0.0 0.0 0.0 0.019903436231958498 0.0 9 0.0 0.0 0.0 0.027607992192716625 0.0 10 0.0 0.0 0.0 0.028250038522779804 0.0 11 0.0 0.0 0.0 0.029534131182906157 0.0 12 0.0 0.0 0.0 0.029534131182906157 0.0 13 0.0 0.0 0.0 0.030818223843032514 0.0 14 0.0 0.0 0.0 0.030818223843032514 0.0 15 0.0 0.0 0.0 0.033386409163285224 0.0 16 0.0 0.0 0.0 0.03531254815347475 0.0 17 0.0 0.0 0.0 0.03595459448353793 0.0 18 0.0 0.0 0.0 0.039806872463916995 0.0 19 0.0 0.0 0.0 0.040448918793980174 0.0 20 0.0 0.0 0.0 0.04301710411423288 0.0 21 0.0 0.0 0.0 0.04943756741486466 0.0 22 0.0 0.0 0.0 0.06163644768606503 0.0 23 0.0 0.0 0.0 0.0706250963069495 0.0 24 0.0 0.0 0.0 0.07640351327751811 0.0 25 0.0 0.0 0.0 0.08153988391802353 0.0 26 0.0 0.0 0.0 0.08603420822846576 0.0 27 0.0 0.0 0.0 0.11300015409111921 0.0 28 0.0 0.0 0.0 0.14253428527402537 0.0 29 0.0 0.0 0.0 0.2035286866300272 0.0 30 0.0 0.0 0.0 0.26837536596640815 0.0 31 0.0 0.0 0.0 0.3428527402537367 0.0 32 0.0 0.0 0.0 0.4160460218809389 0.0 33 0.0 0.0 0.0 0.48025065488725666 0.0 34 0.0 0.0 0.0 0.5778416970568596 0.0 35 0.0 0.0 0.0 0.7351430479223381 0.0 36 0.0 0.0 0.0 0.9367455955621757 0.0 37 0.0 0.0 0.0 1.3232574862602084 0.0 38 0.0 0.0 0.0 1.7097693769582414 0.0 39 0.0 0.0 0.0 2.279906518054343 0.0 40 0.0 0.0 0.0 2.954697210950742 0.0 41 0.0 0.0 0.0 3.7039652781344703 0.0 42 0.0 0.0 0.0 4.5347732292362215 0.0 43 0.0 0.0 0.0 5.350172068416457 0.0 44 0.0 0.0 0.0 6.167497046586882 0.0 45 0.0 0.0 0.0 7.018850480250655 0.0 46 0.0 0.0 0.0 7.884970979505881 0.0 47 0.0 0.0 0.0 8.681750475114285 0.0 48 0.0 0.0 0.0 9.411757152396117 0.0 49 0.0 0.0 0.0 10.200832092043761 0.0 50 0.0 0.0 0.0 10.91992398171452 0.0 51 0.0 0.0 0.0 11.666623863577996 0.0 52 0.0 0.0 0.0 12.540448918793981 0.0 53 0.0 0.0 0.0 13.210103241049874 0.0 54 0.0 0.0 0.0 13.945888335302275 0.0 55 0.0 0.0 0.0 14.70029277312651 0.0 56 0.0 0.0 0.0 15.36352663208177 0.0 57 0.0 0.0 0.0 15.99786840618419 0.0 58 0.0 0.0 0.0 16.693846627972675 0.0 59 0.0 0.0 0.0 17.360290718578252 0.0 60 0.0 0.0 0.0 17.982433612409473 0.0 61 0.0 0.0 0.0 18.612281062201447 0.0 62 0.0 0.0 0.0 19.324952488571576 0.0 63 0.0 0.0 0.0 19.947095382402793 0.0 64 0.0 0.0 0.0 20.624454260619448 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACAA 15 0.002219587 70.01349 68 GGACCTA 15 0.002219587 70.01349 1 ACACGTC 15 0.0022224165 69.99101 6 TCTGGCG 35 2.961806E-7 59.9923 11 CGGTGGT 305 0.0 52.797054 46 CGCTGCG 20 0.0069355257 52.510117 16 TTGCGTG 20 0.0069355257 52.510117 32 CGTCCCA 20 0.0069355257 52.510117 14 TCGGTGG 300 0.0 52.510117 45 TCGCCGT 300 0.0 52.49326 52 CGTGCGA 20 0.006944341 52.49326 10 GGCACGC 20 0.006944341 52.49326 52 GCTCTAT 20 0.006944341 52.49326 3 ATTCAAT 20 0.006944341 52.49326 26 TGGTCGC 310 0.0 51.945488 49 GCCGTAT 300 0.0 51.326744 54 CGTATCA 315 0.0 49.99358 56 GTCGCCG 325 0.0 49.548008 51 CTCGGTG 305 0.0 49.337925 44 GTGGTCG 330 0.0 48.79728 48 >>END_MODULE