FastQCFastQC Report
Wed 25 May 2016
SRR1780835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155752
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT130338.36778981971339No Hit
ATTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT54043.4696183676614103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35052.2503723868714367No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG25761.6539113462427448No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC11030.7081771020596846No Hit
ATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTCTT5950.3820175663875905RNA PCR Primer, Index 38 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC4040.25938671734552365No Hit
CTTATACACATCTCCGCGCCCACGAGACATTTATCTATCTCGTATGCCGT3730.23948328111356518No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC3170.20352868663002724No Hit
ATTATACACATCTCCGCGCCCACGAGACATTTATCTATCTCGTATGCCGT1820.11685243207149827No Hit
ATTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGACGT1630.10465355180029792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC353.6816346E-970.0102848
TAACGTA150.002219990870.0102838
ATATCGT401.3460522E-1070.0102837
ATGACGA207.9013946E-570.0102844
GCGTGCC150.002219990870.0102811
TAACTCG353.6816346E-970.0102836
TCGGTAG150.00222282169.987814
AGGGGGG21450.064.6041270
TATCGTA453.8198777E-1062.23135838
AACTCGT308.338997E-658.34189637
TGCCGTA308.338997E-658.34189645
ATTATAC9800.058.2041441
ATCGTAT553.45608E-1157.2811339
CGTATGA1100.057.26274541
CACGTAT252.3828712E-456.00822439
GTACGTG252.3828712E-456.0082246
AGTACGT252.3866567E-455.9902465
GTATGAC1200.055.4070142
CTGCTTG28500.053.65731454
GCCGTCT29050.053.5018346
Produced by FastQC (version 0.11.5)