FastQCFastQC Report
Wed 25 May 2016
SRR1780834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470919
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7047914.966268084320234No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG35870.7617021186233727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTGT207.92156E-570.003931
AGCGCTT150.002223591670.003932
CGGTGGA452.6977432E-854.44750246
GACGTAT200.0069450452.50852254
GCCGTAA200.0069450452.50852254
GGTACAT200.00694795852.5029451
AAGGTCC200.00694795852.50294545
GGTGGAC407.4719355E-752.50294547
TGGACGC407.4774107E-752.4973749
GTGGACG407.4774107E-752.4973748
TGCGCGT200.006950876752.497375
TGGTCGC5800.051.29053549
TCGGTGG6250.050.9628645
GCCGTAT5800.050.69788754
CTCGGTG6150.050.6586344
TCGCCGT5650.050.18514352
CGGTGGT6350.050.16029446
ATCTCGG6300.050.00811842
GTCGCCG5850.049.65563251
GATCTCG6350.049.6196241
Produced by FastQC (version 0.11.5)