FastQCFastQC Report
Wed 25 May 2016
SRR1780834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470919
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT429209.114093931228089No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG65701.395144387888363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59661.2668845385299807No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT23750.5043330169307249No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC23050.4894684648527666No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT21830.46356167408832516No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT8370.17773757270358598No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC7670.16287302062562775No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG6980.14822081929164038No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT6780.14397380441222377No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT6610.1403638417647196Illumina PCR Primer Index 3 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACTTTAATGCATCTCGTATGCCGT5550.1178546629038115No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTACTGCATCTCGTATGCCGT4970.10553831975350327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAG252.8366485E-670.00722550
AGCGTCA207.9238846E-569.999793
GTCTGCA150.002224113369.9997918
TGACGAC150.002224113369.9997945
CGTCAAC150.002224113369.9997948
AGGGGGG55700.065.92242470
AACTCGT650.064.6151937
CGTCTAC850.061.7645248
TGCATAT800.061.2433134
ATATCGT900.058.3331637
GCATATC900.058.3269635
TGACGTC1700.057.64688545
ATCGTAT850.057.64688539
GCCGTAT553.6379788E-1157.27255246
CGTATGA1800.056.3887241
GCAGCGT252.3883647E-456.005781
GTATTCT750.055.99983249
CAACTCG750.055.99983236
ATCTCGT75850.055.27998737
TCGTATG76250.055.21950540
Produced by FastQC (version 0.11.5)