FastQCFastQC Report
Wed 25 May 2016
SRR1780833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412762
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6286715.230810975816572No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG24850.6020418546280907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACA200.006953845352.49054731
TCGCCGT4600.046.41593652
GCCGTAT4600.046.41593654
TGGTCGC4900.045.71713349
CTCGGTG5050.044.3645844
CGGTGGT5250.044.00807646
GTCGCCG4950.043.84111451
TCGGTGG5200.043.7580345
CGCCGTA5150.042.1385553
CGTATCA5200.041.7333756
ATCTCGG5250.041.33590742
GTAGATC5400.040.84082438
AGATCTC5450.040.4612440
TCTCGGT5550.040.36776743
GGTGGTC5750.040.18128647
GATCTCG5450.039.8238241
GTATCAT5750.038.9589557
CCGTATC5600.038.75241555
TAGATCT5550.038.47086339
CTTTATA654.8530455E-737.6992262
Produced by FastQC (version 0.11.5)