FastQCFastQC Report
Wed 25 May 2016
SRR1780833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412762
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT366218.872183001342178No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG55171.336605598383572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43041.0427316468085726No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC20820.5044068979218048No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT19980.48405618734282707No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT16420.39780793774620726No Hit
CTTATACACATCTCCGCGCCCACGAGACCTCATGGGATCTCGTATGCCGT9740.23597133457052732No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT6690.16207887353971537No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC6300.15263032934233287No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT5530.1339755113116033No Hit
TCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG5430.1315528076712488No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT4510.10926393417998749RNA PCR Primer, Index 34 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTT150.00222453369.994947
GTATCCC150.00222560269.98643542
TCGAAAG207.930216E-569.98643540
CTTAAGC600.064.18532651
AGGGGGG46100.063.92934870
TAAGACG453.8562575E-1062.21015543
ATCGTAA401.065564E-861.2455539
AACTCGT750.060.6622637
GACGACT352.9727562E-759.9956446
AAGCCGT1850.058.64438244
GACGTAT308.374227E-658.32908646
CGTCTTA1750.056.00271248
CGACTTA252.3886639E-456.00271248
TGCCGAC1500.055.9959345
GGATATC2600.055.18829735
ATATCGT2600.055.18829737
CCGTCTT60100.054.33049447
GCCGTCT60900.054.1914646
CGTCTTC59800.054.14142648
TATGCCG64900.054.026543