FastQCFastQC Report
Wed 25 May 2016
SRR1780832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379092
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5457514.396241545587879No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG23770.6270245745096177No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAATC150.002225197269.9885259
CTCGGTG4650.054.1846644
AGATCTC4600.054.02000840
GATCTCG4650.053.43914841
TCGGTGG4850.053.40035245
ATCTCGG4750.053.0439342
TGGTCGC4900.052.8554549
GTCGCCG5150.052.3284251
CGGTGGT4950.052.3215646
GCCGTAT4750.052.3141154
TCTCGGT5050.051.97167643
TCGCCGT4900.051.42692652
GTAGATC4950.050.91417738
TAGATCT5200.048.45998839
CGTATCA5200.047.7869356
CCATAGA305.875316E-446.6651732
CCGTATC5450.046.23705355
GGTGGTC5450.046.23705347
GTATCAT5650.042.74199357
CGGAGTG6150.042.6871932
Produced by FastQC (version 0.11.5)