FastQCFastQC Report
Wed 25 May 2016
SRR1780832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379092
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT319258.421438595380542No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG48301.274097052958121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41431.0928745528789845No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT22130.5837633081151805No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT17020.44896753294714736No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC15760.4157302185221529No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC6370.16803308959302757No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT6280.16565899570552794No Hit
TCATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG5560.1466662446055311No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4980.1313665284416448No Hit
CTTATACACATCTCCGCGCCCACGAGACACCGGCTAATCTCGTATGCCGT4430.11685817690692496No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT4210.11105483629303704TruSeq Adapter, Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT150.00222197270.0141237
GGTCCCA150.002223135270.004881
GCACTAG150.002223135270.004881
TAAGACG207.929393E-569.9864143
AGGGGGG45150.065.2758770
AAGCCGT700.064.9959644
TAACGTA352.9664807E-760.01210438
CTCTCGT850.057.65868837
TCTCGTA58150.057.01063538
CCGTCTT55500.057.00546647
CGTCTTC56250.056.99201248
AATCTCG57450.056.91313636
GCCGTCT56650.056.89848746
AGCCGTC800.056.87146445
ATCTCGT57550.056.75340337
CTCGTAT57800.056.68963239
TATGCCG57600.056.6209543
TCGTATG59350.056.55828540
TCTTCTG56550.056.50450
CGTATGA1550.056.45554741