FastQCFastQC Report
Wed 25 May 2016
SRR1780831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726383
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG539633.125783791893224No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG20040.116080846486556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT3200.038.2860554
TCGCCGT3800.033.16109552
TGGTCGC4300.031.7435749
TCGGTGG4250.030.47087945
CGGTGGT4600.029.67419446
GTCGCCG4600.029.67161651
CGTATCA4250.029.65077856
TCTCGGT4800.027.71100443
CGCCGTA4550.027.6957853
CCGTATC4850.025.9826455
CTCGGTG4850.025.98188844
GTGGTCG5900.023.72835448
CGTACCC800.002665742821.8777433
GTAGATC6400.021.8771138
AACTCGG22950.021.81038370
TAGATCT6750.021.77924739
GATCTCG6100.021.23155641
AAACTCG30300.021.0251370
AGATCTC6750.020.7421440
GGTCATA3050.019.511211
Produced by FastQC (version 0.11.5)