FastQCFastQC Report
Wed 25 May 2016
SRR1780831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726383
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT291891.6907603932615183No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG41110.23812792410490605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34120.19763864681243964No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT19290.1117365034294244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50650.054.65302743
AGGGGGG43700.054.63185570
CGTATGC52000.054.3798241
CCGTCTT47950.054.37476747
GCCGTCT48500.054.33541546
CGTCTTC48300.054.13507548
TCGTATG52650.054.0408240
CTCGTAT51950.053.69268439
TGCCGTC50850.053.13199245
ATCTCGT52250.053.11647437
TCTCGTA52400.053.09800338
ATGCCGT51800.052.9007444
GTATGCC53050.052.24647542
CATCTCG53450.051.20370536
CTTGAAA54900.050.23673257
GTCTTCT51850.049.95606249
CTGCTTG54150.047.9690454
TCTTCTG54150.047.706350
TTGAAAA59500.047.113558
CTTCTGC55450.046.59189651