FastQCFastQC Report
Wed 25 May 2016
SRR1780830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585186
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG532209.094544298735787No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG20490.350145082076468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT252.388622E-456.0068452
GACGTAT200.00694698452.50641354
TACGGGT200.006949332552.50192337
TTCTACG200.00695168252.49743714
TGGTCGA305.8784394E-446.66438749
TGGTCGC4300.045.5791749
GCCGTAT3950.045.1953954
TCGCCGT4100.044.3956652
GTCGCCG4350.044.2469351
CGGTGGT4850.044.0184746
TCGGTGG4750.043.4715645
CTCGGTG4700.043.19307344
CGCCGTA4350.041.0394953
GGTCGAC350.001256092839.9946350
GATCTCG5100.039.1190841
TCTCGGT5400.038.89031243
CGTATCA4800.038.6505556
TAGATCT5350.038.5995539
ATCTCGG5200.038.36351442
GTAGATC5350.037.94531638
Produced by FastQC (version 0.11.5)