FastQCFastQC Report
Wed 25 May 2016
SRR1780830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585186
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT99491.70014320233225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37780.6456066959906765No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT25090.428752567559716No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG11370.19429719781402835No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT6770.1156897123307803No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT6220.10629099124039194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG23100.062.59379270
TTCCGTA1500.060.65822245
TTCCGTC1750.057.99193245
CTCGTAT20300.057.93287739
TCGTATG17900.057.87893340
ATCTCGT22100.057.33213437
TCTCGTA21350.056.8870538
CCGTCTT10100.056.82377247
ATTCCGT3150.056.6587844
CATCTCG24750.056.0017836
TCCGAAA252.391708E-455.99220746
GCCGTGT252.391708E-455.99220746
CGTATGC18200.055.96331841
TATGCCG24550.055.8781743
CGTCTTC5750.055.3883348
GCCGTCA7400.055.3301446
ATGCCGT23100.055.1438444
GTATGCC26850.054.6106542
TACGCGG452.703564E-854.4368729
GCATCTC26350.053.92960735