FastQCFastQC Report
Wed 25 May 2016
SRR1780829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112480
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4917543.71888335704125No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG22652.013691322901849No Hit
TCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGT2850.2533783783783784No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG1540.13691322901849218No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGA150.002218888470.00622629
AGTCATT150.002218888470.00622629
CTTTCAC207.894107E-570.00622619
CGAAACA308.348819E-658.31258425
GTCATTC200.006933372552.50467330
GGCTGCG200.006933372552.50467355
TGCATAA200.00694558552.48132710
TGAATCC200.00694558552.48132740
TATCATT4650.049.70394558
TCGGTGG4800.049.58774645
TGGTCGC4800.049.58774649
GTCGCCG4800.049.56569751
TCGCCGT4600.049.46092252
GCCGTAT4600.049.46092254
GTGGTCG4900.049.290148
CGTATCA4700.049.15330556
GTATCAT4700.049.15330557
CCGTATC4650.048.9290855
CTCGGTG4600.048.69998644
TCTCGGT4650.048.17632743
Produced by FastQC (version 0.11.5)