FastQCFastQC Report
Wed 25 May 2016
SRR1780829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112480
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2671223.748221906116644No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG42983.821123755334282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38223.397937411095306No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT21301.8936699857752488No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT20441.817211948790896No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC15551.3824679943100997No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT13741.2215504978662872No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT5370.47741820768136556No Hit
TCATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG4900.4356330014224751No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC4370.38851351351351354No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT4130.367176386913229No Hit
CTTATACACATCTCCGCGCCCACGAGACGACGCTCCATCTCGTATGCCGT3650.32450213371266No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT3610.32094594594594594Illumina PCR Primer Index 11 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG3330.2960526315789474No Hit
ATAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2450.21781650071123754No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT1810.16091749644381223No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT1770.15736130867709813No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC1690.15024893314366997No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT1540.13691322901849218No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT1500.13335704125177808No Hit
CTAAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1490.1324679943100996No Hit
CAAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1440.12802275960170698No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAGCTCCATCTCGTATGCCGT1370.12179943100995733No Hit
AATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1280.11379800853485066No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC1250.11113086770981509No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTT150.002215476770.0333812
ACACATT150.002215476770.033388
ATTCGTA150.002215476770.0333854
CTGAATG207.878947E-570.0333854
ATATCGA150.002215476770.0333837
GCCTATT150.002215476770.0333835
ACGCCGA150.002219392270.0022227
TCCGCGA150.002219392270.0022213
TGCATTG150.002219392270.0022224
CGAGACT252.8238082E-670.0022223
TACAGGA150.002219392270.002222
GCCCAAG353.6852725E-969.971118
ACGCACC353.6852725E-969.971130
CGACTAA207.91377E-569.971148
GCTGCGT150.002223312669.9710930
CCGTCAA150.002223312669.9710947
GCCGACA252.8312843E-669.9710946
CGAGCAA150.002223312669.9710915
CGTCAAC150.002223312669.9710948
TGCCGAC850.065.8551545
Produced by FastQC (version 0.11.5)