FastQCFastQC Report
Wed 25 May 2016
SRR1780828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60169
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3111451.711013977297284No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG10481.7417607073409895No Hit
TCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGT1220.2027622197477106No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG770.12797287639814522No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750.12464890558260898No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710.1180009639515365No Hit
ATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTGGTC650.10802905150492778No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAAG150.002214640670.025
AAATAGA150.002214640670.02
TGGCGCT150.002214640670.013
TACCATC150.002214640670.063
GTCGGCG150.002214640670.051
GGTCGGC150.002214640670.050
TGGCGGC252.3728034E-455.99999619
TCTGGCG200.006920210652.511
GGCCGGC200.006920210652.519
CTACCAT200.006920210652.562
TGGTCGC2500.050.39999849
CGCCGTA2400.049.58333253
CGGTGGT2550.049.41176646
TCGGTGG2700.049.2592645
CCGTATC2350.049.14893755
TCGCCGT2350.049.14893752
GCCGTAT2350.049.14893754
GTCGCCG2500.049.051
GGTGGTC2650.048.8679247
GGTCGCC2550.048.03921550
Produced by FastQC (version 0.11.5)