FastQCFastQC Report
Wed 25 May 2016
SRR1780828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60169
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1819630.24148647974871No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG29694.934434675663548No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20793.455267662749921No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT14142.3500473665841213No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT11341.884691452409048No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC9391.5606042978942645No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4560.7578653459422626No Hit
TCATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG3910.6498362944373348No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3700.6149346008742044No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3180.5285113596702621No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT2880.47865179743721853TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT2850.4736658412139142No Hit
CTTATACACATCTCCGCGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2040.3390450231846964No Hit
ATAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1640.27256560687397163No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT1600.26591766524289917No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC1310.2177200884176237No Hit
CAAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1240.20608619056324684No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1130.18780435107779753No Hit
AATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT970.16121258455350762No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC970.16121258455350762No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCG920.15290265751466703No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT850.14126875966029018No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG850.14126875966029018No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG820.13628280343698582No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC800.13295883262144959No Hit
ATTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT710.1180009639515365No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAATCTCGTATGCCGTC700.11633897854376837No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCAGT680.11301500772823216No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT680.11301500772823216No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC650.10802905150492778No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGT150.002209211270.0432625
TTGATAA150.002209211270.0432658
CTTGATA150.002209211270.0432657
CGAGACT150.002209211270.0432623
AGACTGA150.002209211270.0432625
TCAAGTA150.002216518469.9850438
ACGCTGT150.002216518469.9850440
ATCTCGA500.069.9850437
CATTCTC150.002216518469.9850414
GTATCAG150.002216518469.985041
TATCAGG150.002216518469.985042
CTTAAAC700.069.985041
CTGCCGT150.002216518469.9850444
GCCGTAA150.002216518469.9850446
TTAATAC150.002216518469.985043
AATGCCG252.811943E-669.9850443
CAAGTAT150.002216518469.9850439
ACATCAA207.876124E-569.985038
AAAACAG207.876124E-569.9850365
AACACAT2450.067.12855
Produced by FastQC (version 0.11.5)