FastQCFastQC Report
Wed 25 May 2016
SRR1780827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827336
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG551926.67105021418142No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG25950.3136573290658209No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTC150.00222532369.9949342
GCCGTAT4550.045.3868154
TGGTCGC4950.043.83785249
CGGTGGT5050.043.66283446
TCGGTGG5250.043.3328145
CTCGGTG5000.042.69948244
TCGCCGT4950.042.42629252
GTCGCCG5050.041.58114251
TCTCGGT5750.038.9560543
CGTATCA5400.038.8907756
GTAGATC5900.037.96564538
GATCTCG5750.037.74095541
CGCCGTA5600.037.50181253
ATCTCGG5800.036.80767442
AGATCTC6000.036.16841540
TAGATCT6150.035.28626339
GTGGTCG6100.034.99957748
GTATCAT6050.034.1338857
CCGTATC6200.033.30806455
GGGTCTA851.0650729E-732.938791
Produced by FastQC (version 0.11.5)