FastQCFastQC Report
Wed 25 May 2016
SRR1780827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827336
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT300893.636853708771285No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG46220.5586605683785064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43320.5236083042439831No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC16710.20197356334065No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT14340.17332740265140162No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT11530.13936296740381177No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT11500.13900035777483394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG37300.058.28119370
CCGTCTT43900.053.41026347
GCCGTCT43700.053.33437346
TATGCCG52900.052.92352343
CGTCTTC40500.052.61995348
ACGCCGT200.006948100452.5062138
TCATACG200.006954747352.4935247
CGTATGC50900.052.47182541
GTATGCC53550.052.34647842
ATGCCGT53150.052.3453744
CGTCTTA5150.052.32047748
TCGTATG52500.052.2728540
CTCGTAT52900.051.94376439
TGCCGTC50550.051.8531445
TCTCGTA55000.051.74248538
ATCTCGT55250.051.50835437
TGCCGTA3350.051.1877145
TCTTCTG38050.050.86150
CTTGAAA47000.050.79324357
GTATCTC56250.050.65181735
Produced by FastQC (version 0.11.5)