FastQCFastQC Report
Wed 25 May 2016
SRR1780826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304184
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4633015.230912868526945No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG20700.680509165505089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC3450.052.75077449
GCTCTCG200.00694411352.505841
TCACTAC200.00694411352.505840
CCGTAAC200.006948630352.49716655
CTCTCGG200.006948630352.49716642
CCGTTGT200.006948630352.49716658
GCCGTAA200.006948630352.49716654
TCGCCGT3250.050.61265652
GTCGCCG3600.050.55282251
CGGTGGT3700.050.13242746
CTCGGTG3650.049.86031744
TCGGTGG3900.049.35630845
GCCGTAT3300.047.72469754
GATCTCG3550.047.3291741
CGCCGTA3550.047.3213953
GTAGATC3850.047.27794638
CCGCGTC305.8693526E-446.67181817
TTAGCAC305.874112E-446.6641464
CGGTGGA305.874112E-446.66414646
TCTCGGT4000.046.37249443
Produced by FastQC (version 0.11.5)