FastQCFastQC Report
Wed 25 May 2016
SRR1780826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304184
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT271278.917957552007994No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG42651.4021118796517897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35141.155221839412987No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT19670.646648081424401RNA PCR Primer, Index 19 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC16210.5329011387844199No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT14010.46057649317518345RNA PCR Primer, Index 19 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT5590.1837703495252873No Hit
TCATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG5100.16766167845777555No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC4430.1456355363858717No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4420.14530678799673882RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3570.11736317492044288No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT3360.11045945874865212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAT150.002223016570.00246427
GAACACG252.8353752E-670.00246418
AGGGGGG38350.063.24864670
ATCGTAT850.057.64909439
CCGTCTT48950.052.8414947
TCGTATG52100.052.80416540
CGTATGC51000.052.7763741
TATGCCG50150.052.69378343
TCTCGTA51550.052.6885738
GTATGCC50450.052.65794842
CTCGTAT51450.052.6549239
CGTCTTC49800.052.63376248
ATCTCGT50550.052.62302437
GCCGTCT49300.052.60834546
GGAATTC200.00694617952.50184638
CATTCGG200.00694617952.50184645
GCCGTAT407.4641866E-752.50184646
TGTCAAT200.006950697452.49321710
GCTCTAT200.006950697452.4932179
CTCTATG200.006950697452.49321710
Produced by FastQC (version 0.11.5)