FastQCFastQC Report
Wed 25 May 2016
SRR1780825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94749
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6185365.28090006226978No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG30073.1736482706941493No Hit
TCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGT3590.3788958194809444No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1890.19947440078523257No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG1550.1635901170460902No Hit
ATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTGGTC1290.13614919418674604No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1260.13298293385682172No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1230.12981667352689738No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1180.12453957297702349No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1100.1160962120972253No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1030.10870827132740186No Hit
TCTTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG950.10026491044760368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG150.002212413470.049162
CTGATGG150.002212413470.0491652
CGAATCA150.002212413470.0491656
CGCGGTA150.002212413470.0491653
GCCGAAT150.002212413470.0491654
GTTGCCA150.002212413470.049161
CCGAATC150.002212413470.0491655
TTATACG207.86374E-570.049152
ACAGACG150.00221705870.0121511
TTTGGGG207.884368E-570.0121463
CAACAGA150.0022217169.975199
GGTAGGT150.0022217169.9751937
AAACGTC150.0022217169.975196
TTTGCGG150.0022217169.9751931
GGTCGGC150.0022217169.9751950
GGGCGCT352.943143E-760.01041413
ACTCGGA5100.058.99868429
CCGTATC5150.058.48764455
GCCGTAT5150.058.48764454
CGTATCA5100.058.37429456
Produced by FastQC (version 0.11.5)