##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780825_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94749 Sequences flagged as poor quality 0 Sequence length 76 %GC 87 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.18526844610497 32.0 27.0 32.0 13.0 32.0 2 28.37572955915102 32.0 27.0 32.0 13.0 32.0 3 28.272636122808684 32.0 27.0 32.0 13.0 32.0 4 27.638750804757834 32.0 27.0 32.0 13.0 32.0 5 28.21708936242071 32.0 27.0 32.0 13.0 32.0 6 31.963366367982776 37.0 32.0 37.0 13.0 37.0 7 31.622412901455423 37.0 32.0 37.0 13.0 37.0 8 32.0583858404838 37.0 32.0 37.0 13.0 37.0 9 32.172930585019365 37.0 32.0 37.0 13.0 37.0 10-11 31.924695775153296 37.0 32.0 37.0 13.0 37.0 12-13 31.733205627500027 37.0 32.0 37.0 13.0 37.0 14-15 32.323201300277574 37.0 34.5 37.0 13.0 37.0 16-17 32.64969023419772 37.0 34.5 37.0 13.0 37.0 18-19 32.19526855164698 37.0 32.0 37.0 13.0 37.0 20-21 32.608513018607056 37.0 34.5 37.0 13.0 37.0 22-23 32.69817095694941 37.0 34.5 37.0 13.0 37.0 24-25 32.12097753010586 37.0 32.0 37.0 13.0 37.0 26-27 32.6672207622244 37.0 34.5 37.0 13.0 37.0 28-29 32.35891143969857 37.0 32.0 37.0 13.0 37.0 30-31 32.403787902774695 37.0 32.0 37.0 13.0 37.0 32-33 32.552211632840454 37.0 32.0 37.0 13.0 37.0 34-35 32.33360246546138 37.0 32.0 37.0 13.0 37.0 36-37 33.104803216920494 37.0 34.5 37.0 13.0 37.0 38-39 33.17817602296594 37.0 34.5 37.0 17.5 37.0 40-41 32.643510749453824 37.0 34.5 37.0 13.0 37.0 42-43 32.43357713537874 37.0 32.0 37.0 13.0 37.0 44-45 32.30185015145278 37.0 32.0 37.0 13.0 37.0 46-47 32.63673495234778 37.0 34.5 37.0 13.0 37.0 48-49 33.1444289649495 37.0 34.5 37.0 13.0 37.0 50-51 33.4310124645115 37.0 37.0 37.0 13.0 37.0 52-53 32.577911112518336 37.0 32.0 37.0 13.0 37.0 54-55 32.76068876716377 37.0 34.5 37.0 13.0 37.0 56-57 32.59307222239813 37.0 34.5 37.0 13.0 37.0 58-59 32.146677009783744 37.0 32.0 37.0 13.0 37.0 60-61 31.943519192814698 37.0 32.0 37.0 13.0 37.0 62-63 31.575087863724157 37.0 32.0 37.0 13.0 37.0 64-65 31.333285839428385 37.0 29.5 37.0 13.0 37.0 66-67 31.281058375286282 37.0 32.0 37.0 13.0 37.0 68-69 31.548259084528596 37.0 32.0 37.0 13.0 37.0 70-71 31.796372521082017 37.0 32.0 37.0 13.0 37.0 72-73 32.13918352700293 37.0 32.0 37.0 13.0 37.0 74-75 32.16417587520713 37.0 32.0 37.0 13.0 37.0 76 32.867966944242156 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.910875108581326 11101 2 0.6036052138571719 11101 3 0.9494244476958968 11101 4 0.7956833104114267 11101 5 -0.8354411807701183 11101 6 1.2832681440076712 11101 7 0.830871034599781 11101 8 0.8902465276870863 11101 9 0.06816931721741071 11101 10-11 1.58153611679149 11101 12-13 0.03368167468737937 11101 14-15 -0.7154783562937794 11101 16-17 0.19777392933238502 11101 18-19 -0.5970046800664193 11101 20-21 0.4106455302732428 11101 22-23 -0.022241121035854405 11101 24-25 -0.3198323412170545 11101 26-27 -0.09408821593129346 11101 28-29 -0.33449596131782755 11101 30-31 0.8289056384576838 11101 32-33 0.16245348571727192 11101 34-35 -0.5993211115039365 11101 36-37 0.07615640428893755 11101 38-39 -0.23496369159358466 11101 40-41 -0.04579200585054366 11101 42-43 0.22994772178090273 11101 44-45 -0.3581033689114719 11101 46-47 0.20735097628708132 11101 48-49 -0.35986129440641434 11101 50-51 -0.2497828135247957 11101 52-53 -0.4089133359061208 11101 54-55 0.6192699638827364 11101 56-57 -0.20028762605302575 11101 58-59 1.0005176880862479 11101 60-61 1.512816870295886 11101 62-63 -0.3780389845612717 11101 64-65 0.7538752306306549 11101 66-67 0.09400160108387112 11101 68-69 0.7297868461716277 11101 70-71 -0.3803115550796754 11101 72-73 -0.40960092827694794 11101 74-75 -1.7214809690517612 11101 76 -2.3185959946003223 11102 1 -0.7358974324022789 11102 2 -1.1730341304051244 11102 3 -0.15200997853361287 11102 4 -0.37363021417873554 11102 5 0.3264440651315219 11102 6 -0.971342511730036 11102 7 1.2225206247637175 11102 8 0.11975472440839852 11102 9 0.2651467762338058 11102 10-11 0.5970330430209927 11102 12-13 -0.05878758760770353 11102 14-15 0.40055647977179376 11102 16-17 -1.5047107018151564 11102 18-19 0.24085392649095638 11102 20-21 -0.29683397792347677 11102 22-23 -0.2526458341506057 11102 24-25 -1.4938845953154143 11102 26-27 -0.4921414946198155 11102 28-29 -0.32796419902274465 11102 30-31 -0.5107460008865772 11102 32-33 -0.351890776577811 11102 34-35 -1.3684041033072134 11102 36-37 -0.6081417514487697 11102 38-39 -0.011218814544399436 11102 40-41 -0.22547950585054366 11102 42-43 -1.6348063765797463 11102 44-45 -0.022293942681962164 11102 46-47 -0.8971572204342309 11102 48-49 -0.6901635485047777 11102 50-51 -1.0453770758198786 11102 52-53 1.0211583854053572 11102 54-55 -0.22268700333037827 11102 56-57 -0.7625314785120452 11102 58-59 -0.6380581315858791 11102 60-61 0.9521099030827713 11102 62-63 0.08148970396332089 11102 64-65 -0.3480714906808231 11102 66-67 -2.238221759571868 11102 68-69 -0.5544088505496845 11102 70-71 -0.38863635016164366 11102 72-73 -1.3747648627031772 11102 74-75 -2.2833406001993026 11102 76 -1.093954601157698 11103 1 -0.7120757110908045 11103 2 -0.6259029828641403 11103 3 -0.11614932279590917 11103 4 -0.8764478371295539 11103 5 0.7055424257872609 11103 6 -0.6347646428775775 11103 7 1.0275923460751955 11103 8 -1.3228682264112734 11103 9 0.21571030082397158 11103 10-11 -1.3446933914052366 11103 12-13 -0.06467898105032788 11103 14-15 -0.06793737268722566 11103 16-17 0.23875753588976067 11103 18-19 -0.47405386039428876 11103 20-21 -0.30246922382511343 11103 22-23 -0.15338866201945933 11103 24-25 -0.7788487346596753 11103 26-27 -0.9793341175706374 11103 28-29 -0.31810251869487516 11103 30-31 -0.4743730500669052 11103 32-33 -0.468694055266333 11103 34-35 -1.107517832815411 11103 36-37 -0.12056490718647694 11103 38-39 -0.005455494872272482 11103 40-41 -0.20972643208005337 11103 42-43 -0.6471014585469561 11103 44-45 0.6255031884655793 11103 46-47 0.2155476975985522 11103 48-49 -0.8516645730949435 11103 50-51 -0.331750026639547 11103 52-53 -0.8925198932831648 11103 54-55 -0.07745134759267103 11103 56-57 -1.052746642446472 11103 58-59 0.615271786446911 11103 60-61 -0.14292083462214578 11103 62-63 -0.9354160337415962 11103 64-65 -0.5576001792054157 11103 66-67 -0.11911315301449221 11103 68-69 -0.5898852849759137 11103 70-71 -0.5934263091780387 11103 72-73 -1.655502567621209 11103 74-75 -0.20508752642881234 11103 76 -0.38416976509212475 11104 1 -0.3059867181165643 11104 2 0.9448230124520194 11104 3 0.28549002146638713 11104 4 0.4027090715355506 11104 5 0.29430120798866355 11104 6 -0.5722353688728923 11104 7 -1.080604375236284 11104 8 0.4911832958369722 11104 9 0.2798789190909474 11104 10-11 0.8526134001638539 11104 12-13 -0.5556625876077028 11104 14-15 0.29564576548607846 11104 16-17 0.6923875124705603 11104 18-19 0.07255035506238983 11104 20-21 -0.17272683506633513 11104 22-23 0.8172202372779651 11104 24-25 -0.511518523886842 11104 26-27 0.5569656482373233 11104 28-29 1.2477054438343984 11104 30-31 1.3472897133991424 11104 32-33 0.5016806519936168 11104 34-35 0.16976553954992823 11104 36-37 -0.11506139430591134 11104 38-39 -0.26412060025868556 11104 40-41 -0.9600777201362547 11104 42-43 -0.6776635194368907 11104 44-45 0.634402485889467 11104 46-47 0.3849856367086275 11104 48-49 -0.44440461993335134 11104 50-51 0.4965872098944075 11104 52-53 0.29928338540536004 11104 54-55 1.2241879966696203 11104 56-57 0.12184352148795341 11104 58-59 -0.1938617030144485 11104 60-61 0.3963063316542019 11104 62-63 -0.3747602960366798 11104 64-65 0.6242499378906032 11104 66-67 0.6470460975709891 11104 68-69 -0.07114992197825387 11104 70-71 0.6397118641240702 11104 72-73 0.12166370872539645 11104 74-75 -1.0268673859135902 11104 76 -1.9452938868719833 11105 1 0.4622672501374048 11105 2 -0.10755793992893459 11105 3 -0.5160972801209134 11105 4 -1.7369734681469886 11105 5 0.506999620687079 11105 6 -0.5135052101427355 11105 7 -1.3218742165061244 11105 8 -0.7850071803535066 11105 9 -1.1582163190042891 11105 10-11 -1.1513548538044063 11105 12-13 -0.7802657622108811 11105 14-15 0.521836241676553 11105 16-17 0.5606414807245272 11105 18-19 -0.7941163116042809 11105 20-21 0.5002890379495355 11105 22-23 0.6576964277541535 11105 24-25 0.07816401579569998 11105 26-27 0.9061719974436784 11105 28-29 0.7810387771677298 11105 30-31 -1.2265198104103874 11105 32-33 -0.6753034749905069 11105 34-35 -0.37150430171990934 11105 36-37 -0.7285534577979718 11105 38-39 1.0858793997413159 11105 40-41 -0.27595073600927833 11105 42-43 -0.3927428845162524 11105 44-45 0.2740850255720062 11105 46-47 0.009588811311800782 11105 48-49 -0.041230016758746046 11105 50-51 0.7616665749737663 11105 52-53 -0.7023039161819469 11105 54-55 0.5781562506378748 11105 56-57 0.2258117754562079 11105 58-59 -0.1462426553954046 11105 60-61 -0.599725414377545 11105 62-63 -0.32079204206842604 11105 64-65 0.99964676328743 11105 66-67 0.43514133566622704 11105 68-69 -0.4362292870576212 11105 70-71 -0.9071135327013273 11105 72-73 -0.03944740238571853 11105 74-75 -0.033216592262796496 11105 76 2.188039446461353 11106 1 0.20595707579444067 11106 2 0.5216380007424171 11106 3 -1.5095919457467275 11106 4 -1.0075953781131624 11106 5 -0.5403592135570037 11106 6 0.8898255210568493 11106 7 -0.3822437194985788 11106 8 -0.1917206854276685 11106 9 1.2648906286928252 11106 10-11 -0.41026716189703905 11106 12-13 -0.17943307941098396 11106 14-15 -1.8466258972773844 11106 16-17 0.025642781791404445 11106 18-19 -0.24454566367298014 11106 20-21 -1.7778790598906902 11106 22-23 -1.0222411210358509 11106 24-25 -0.6640946362990192 11106 26-27 -1.0039242815050606 11106 28-29 -0.1295779285309422 11106 30-31 -0.9087992795750992 11106 32-33 0.04769938735661583 11106 34-35 0.48264610161082544 11106 36-37 -0.6369583498094258 11106 38-39 -0.02184893749522132 11106 40-41 0.18371619087076851 11106 42-43 0.2955214922727123 11106 44-45 -0.5384312377639304 11106 46-47 -0.8172391876473455 11106 48-49 -0.2860908026031339 11106 50-51 0.05349587499979691 11106 52-53 -0.31055268016841353 11106 54-55 -0.38073003611726364 11106 56-57 0.455450078865006 11106 58-59 -1.0568593610940766 11106 60-61 -0.7576749329828019 11106 62-63 -1.1895143943973352 11106 64-65 -1.1149772283857402 11106 66-67 0.61039504370682 11106 68-69 -0.19644266202509542 11106 70-71 -0.0196558173747583 11106 72-73 -1.2128796168015405 11106 74-75 -0.3854153952812709 11106 76 0.05845318572755076 11107 1 0.5539211159848172 11107 2 0.008417482498099815 11107 3 -0.9564454624045808 11107 4 0.0976399471115883 11107 5 0.83854083932507 11107 6 -1.073157027859068 11107 7 0.26133110863468545 11107 8 -1.7028259207528933 11107 9 -1.1113653205403864 11107 10-11 -1.431948811817719 11107 12-13 -0.5572754908335114 11107 14-15 -0.8045846492604625 11107 16-17 -0.8235111050409643 11107 18-19 0.3875273135877322 11107 20-21 -0.18493881663315648 11107 22-23 0.4280497303655224 11107 24-25 -1.460366450154126 11107 26-27 -0.7229882688133671 11107 28-29 -1.2193452474098407 11107 30-31 -0.4573185815317373 11107 32-33 -0.7994714217390992 11107 34-35 -1.0279303129846333 11107 36-37 -0.6754300579003853 11107 38-39 -0.5800192177702073 11107 40-41 -0.29579200585054366 11107 42-43 -0.046096699160386834 11107 44-45 -0.2909431362303465 11107 46-47 -2.094737865595519 11107 48-49 -0.9681373388273613 11107 50-51 0.40119550482528865 11107 52-53 -1.449103711368835 11107 54-55 -1.2725861968787626 11107 56-57 0.44764997310085874 11107 58-59 -1.501465389650395 11107 60-61 -1.120974774336581 11107 62-63 -0.8892764250689353 11107 64-65 0.269872057706273 11107 66-67 0.12216130494426025 11107 68-69 0.20995606880515538 11107 70-71 -1.0310254630648679 11107 72-73 -0.7497648627031772 11107 74-75 -1.1782498743928507 11107 76 -0.7144183108351143 11108 1 0.9489583368284897 11108 2 -0.32880336117435505 11108 3 0.8931823291586944 11108 4 1.2400717088981885 11108 5 0.45913637282383135 11108 6 0.7541382575007347 11108 7 2.196318701686792 11108 8 2.25821626286994 11108 9 3.0447140839261166 11108 10-11 2.12239361994407 11108 12-13 1.9789527970076861 11108 14-15 1.6060853259256405 11108 16-17 0.6242556443386889 11108 18-19 1.397825080337114 11108 20-21 0.40749294515344303 11108 22-23 0.5880993581570877 11108 24-25 0.6593605969922791 11108 26-27 0.8294931207647949 11108 28-29 1.0125406086695605 11108 30-31 0.7291578452672667 11108 32-33 1.023109223422189 11108 34-35 0.6884468582312451 11108 36-37 0.3470265177819982 11108 38-39 1.010055223917142 11108 40-41 -0.19194585200439462 11108 42-43 1.2382705464971764 11108 44-45 0.6366002880872728 11108 46-47 1.031688933411921 11108 48-49 1.773177797649069 11108 50-51 0.10318061648781196 11108 52-53 0.6915910777130563 11108 54-55 1.031880304361934 11108 56-57 0.4256896753341124 11108 58-59 0.7621822530295077 11108 60-61 0.06388874923661803 11108 62-63 0.8098550885787077 11108 64-65 1.7610631247037958 11108 66-67 1.9783647788896737 11108 68-69 0.4387401879118542 11108 70-71 0.6831184575306644 11108 72-73 0.7156197526814445 11108 74-75 -0.3103838694300727 11108 76 -0.5211180626961607 11109 1 0.08881847668862974 11109 2 -0.6798523122233071 11109 3 0.6218536578300231 11109 4 1.0260856949121724 11109 5 -0.5212832075957508 11109 6 0.13036203372450927 11109 7 0.3012138065818988 11109 8 0.1743001789538532 11109 9 0.24611268532471797 11109 10-11 1.1363796339300833 11109 12-13 0.7125192305741166 11109 14-15 1.387204207044519 11109 16-17 0.5781017981848464 11109 18-19 -0.3196574371454055 11109 20-21 0.14805238571288726 11109 22-23 0.59579166584939 11109 24-25 0.6306892683209497 11109 26-27 -0.08589149461981549 11109 28-29 0.20484830097726103 11109 30-31 -0.2715414554320361 11109 32-33 1.1049274052403675 11109 34-35 0.21781748760187725 11109 36-37 0.18688665764214107 11109 38-39 0.8715936854556006 11109 40-41 0.4996625396040031 11109 42-43 0.7242845325111631 11109 44-45 -0.43962348813650465 11109 46-47 1.3778427795657677 11109 48-49 0.30884213331339794 11109 50-51 0.7381456514528466 11109 52-53 0.8811015672235385 11109 54-55 1.278733451215075 11109 56-57 0.44457079421522394 11109 58-59 1.204839595686849 11109 60-61 -1.0095378241899553 11109 62-63 -0.774760296036682 11109 64-65 1.0093148729555423 11109 66-67 -0.31464221411732396 11109 68-69 0.7730059221775889 11109 70-71 0.5208806952929059 11109 72-73 -0.13612849906681035 11109 74-75 0.35884689980069595 11109 76 -0.6232159647940634 11110 1 -0.00815122028106785 11110 2 -0.040458372829366596 11110 3 -2.1993584633820973 11110 4 -1.755732486906009 11110 5 -0.9727983591109037 11110 6 -0.8908500874876104 11110 7 -0.28363467826658706 11110 8 -0.9105483058946326 11110 9 0.05823389744593044 11110 10-11 -1.0424082448577963 11110 12-13 -1.1662686482137659 11110 14-15 -1.9673412475009329 11110 16-17 -1.5461406260575785 11110 18-19 -1.146930164418137 11110 20-21 -1.2731597354992346 11110 22-23 -0.8390568189990901 11110 24-25 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-1.0075338505496845 21611 70-71 -1.7870738501616437 21611 72-73 -1.4138273627031772 21611 74-75 -0.9083406001993026 21611 76 0.015420398842302063 21612 1 0.36037272888804495 21612 2 0.28261103088519945 21612 3 0.9145222795309031 21612 4 1.5492528503373926 21612 5 0.19337954900248988 21612 6 1.2655526495602842 21612 7 1.0516536892798456 21612 8 1.2003998856987188 21612 9 1.3241185504273574 21612 10-11 1.9390189301177685 21612 12-13 0.9265954769084246 21612 14-15 1.340576641062114 21612 16-17 1.466811475604203 21612 18-19 1.1536563458457962 21612 20-21 1.194093441431363 21612 22-23 1.1377271497203623 21612 24-25 1.1606012917813615 21612 26-27 1.8979794731221205 21612 28-29 1.5790418493643514 21612 30-31 1.5426814184682627 21612 32-33 2.071496320196381 21612 34-35 0.6494890418540749 21612 36-37 1.8729570388738068 21612 38-39 1.468367879003985 21612 40-41 2.357433800601072 21612 42-43 1.6313226556783178 21612 44-45 2.5155084766728706 21612 46-47 1.3246169731141535 21612 48-49 1.6850884676242543 21612 50-51 1.8528084080510894 21612 52-53 2.4379930628247166 21612 54-55 1.920962190218006 21612 56-57 1.0686177150363392 21612 58-59 2.869502352285089 21612 60-61 1.2661219998569635 21612 62-63 2.2800784136407444 21612 64-65 1.044065606093369 21612 66-67 1.557645175912004 21612 68-69 1.4518915526761234 21612 70-71 1.5738132466125485 21612 72-73 1.6292673953613352 21612 74-75 1.112072706252313 21612 76 1.3017107214229497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 16.0 12 81.0 13 189.0 14 406.0 15 628.0 16 942.0 17 1296.0 18 1391.0 19 1406.0 20 1565.0 21 1581.0 22 1722.0 23 1673.0 24 1688.0 25 1684.0 26 1799.0 27 1910.0 28 2152.0 29 2317.0 30 2760.0 31 3266.0 32 4115.0 33 5575.0 34 8398.0 35 15094.0 36 31094.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 83.2047452689795 3.998015768309287 2.8085321962700664 9.988706766441153 2 79.44460862957021 4.363336992316135 12.042978974921894 4.149075403191759 3 78.96047964870799 4.548429319371728 12.574945110623206 3.9161459212970784 4 79.2008358927271 9.393238978775502 6.662867154270757 4.743057974226641 5 78.71006554158882 6.5742118650328765 12.376911629674192 2.3388109637041024 6 81.95653779987124 11.885085858425946 3.6327560185331773 2.5256203231696377 7 80.83105533684444 6.601792141175975 3.4977360760762877 9.0694164459033 8 81.56181067874067 11.641283813021772 3.1050459635457894 3.6918595446917646 9 79.72115800694466 4.617462981139642 2.9921160117784886 12.669263000137205 10-11 81.14956965017916 7.94366137722497 6.966337197950363 3.940431774645509 12-13 80.28243340228818 5.2117195085912105 7.496728163127455 7.00911892599316 14-15 84.3694259448848 6.749554075590784 4.23549030576166 4.645529673762757 16-17 83.76204599909227 5.226353954465332 3.605091776527586 7.40650826991482 18-19 80.40096256345848 5.069289792816659 6.873568556260357 7.656179087464511 20-21 83.9304467136336 4.7563260244333625 4.440750415578248 6.872476846354784 22-23 83.85392754426239 4.761075490118473 3.553128050872054 7.831868914747091 24-25 81.73873579110685 7.666205790156945 3.491403421744224 7.10365499699199 26-27 84.37389510124171 7.583761220494257 3.703488709583793 4.338854968680243 28-29 81.9290968770119 10.419107325670982 3.8945002058069216 3.7572955915102004 30-31 81.57130945973044 5.978954923007103 6.105077626149089 6.344657991113363 32-33 83.50897375684048 4.961556119621947 4.727251618759136 6.80221850477844 34-35 84.44480798763016 7.093665729077063 4.307283118637628 4.154243164655159 36-37 84.59926856684487 5.053010433213187 7.085296927030835 3.2624240729111147 38-39 84.58648203656014 4.592709080930469 7.600688141174484 3.2201207413349096 40-41 84.27402118328769 7.449508942471594 4.934851099536121 3.3416187747046 42-43 81.9122101552523 7.461820177521663 7.162608576343814 3.4633610908822257 44-45 81.23713625281025 4.754968704811965 7.444349450619043 6.563545591758759 46-47 83.77961897725473 4.30787904374901 4.8920787376642565 7.020423241331997 48-49 84.83089442049214 4.1752639883481 7.2766323477416535 3.717209243418101 50-51 87.23990353409289 4.051779183839322 5.076069805854446 3.632247476213344 52-53 81.56828184533548 4.631808922709954 7.235585295576641 6.564323936377933 54-55 84.03879744798071 4.498751959133918 4.396903381056164 7.065547211829211 56-57 83.7311534584756 4.741122269659245 4.127900533534574 7.39982373833058 58-59 81.69002934408579 7.435242458147734 6.99455339990289 3.8801747978635817 60-61 80.76285765548977 5.407444933455762 7.31089510179527 6.5188023092592005 62-63 80.7157896958791 8.151580236098724 6.8280764339277145 4.3045536340944714 64-65 80.59094219342036 8.13007271998058 7.679398820015409 3.5995862665836382 66-67 80.6522771650219 11.513536334371206 4.011293471951027 3.8228930286558658 68-69 80.7066335954404 11.9409995250409 3.4423980157264236 3.909968863792285 70-71 81.10995007546732 11.558637577446355 3.4667468836747832 3.864665463411545 72-73 81.99654875802783 10.724708042871391 3.473933624279012 3.804809574821765 74-75 84.35814626011884 8.3974501050143 3.418505736208298 3.8258978986585612 76 88.0463118344257 4.8306578433545475 3.2644144001519804 3.858615922067779 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 3.0 8 6.0 9 6.0 10 7.5 11 12.0 12 15.0 13 14.5 14 18.5 15 30.5 16 41.5 17 45.0 18 49.5 19 67.5 20 78.0 21 75.0 22 89.5 23 110.0 24 126.0 25 136.0 26 185.0 27 225.0 28 237.5 29 259.0 30 267.5 31 299.5 32 338.5 33 354.0 34 383.0 35 412.5 36 434.0 37 455.0 38 505.0 39 579.0 40 632.0 41 688.5 42 716.0 43 850.5 44 1672.5 45 2624.0 46 2888.0 47 1968.0 48 928.5 49 709.5 50 610.0 51 562.0 52 516.5 53 495.5 54 472.0 55 446.5 56 433.5 57 372.5 58 299.0 59 307.5 60 284.0 61 248.0 62 244.0 63 215.5 64 185.0 65 179.5 66 165.0 67 154.0 68 159.5 69 168.5 70 167.5 71 163.0 72 165.0 73 172.0 74 171.5 75 166.0 76 177.0 77 183.0 78 189.0 79 200.0 80 203.0 81 211.5 82 234.0 83 251.0 84 258.0 85 275.0 86 277.0 87 269.0 88 302.0 89 364.0 90 438.0 91 525.5 92 568.0 93 599.0 94 763.0 95 1030.0 96 1164.0 97 1457.5 98 2584.5 99 33188.5 100 62959.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002110840219949551 2 0.005277100549873877 3 0.01372046142967208 4 0.0 5 0.0 6 0.0 7 0.002110840219949551 8 0.0 9 0.0 10-11 5.277100549873877E-4 12-13 0.0010554201099747754 14-15 0.002110840219949551 16-17 0.008443360879798203 18-19 0.002110840219949551 20-21 0.0015831301649621632 22-23 0.0015831301649621632 24-25 0.002110840219949551 26-27 5.277100549873877E-4 28-29 0.0 30-31 0.0 32-33 5.277100549873877E-4 34-35 0.0026385502749369386 36-37 0.003693970384911714 38-39 0.0010554201099747754 40-41 0.0047493904948864895 42-43 0.0 44-45 0.006332520659848653 46-47 0.004221680439899102 48-49 5.277100549873877E-4 50-51 5.277100549873877E-4 52-53 0.002110840219949551 54-55 5.277100549873877E-4 56-57 0.003693970384911714 58-59 0.01160962120972253 60-61 0.0 62-63 5.277100549873877E-4 64-65 0.002110840219949551 66-67 0.004221680439899102 68-69 0.004221680439899102 70-71 0.006332520659848653 72-73 5.277100549873877E-4 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 94749.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.79128011905139 #Duplication Level Percentage of deduplicated Percentage of total 1 92.57829828764085 22.02556227506359 2 3.88607931860527 1.8490960326758066 3 1.481678644308402 1.057530950194725 4 0.802945612634194 0.7641241596217374 5 0.3504569248513885 0.4168909434400363 6 0.13308490817141336 0.1899756197954596 7 0.07541478129713425 0.12559499308699829 8 0.07541478129713425 0.14353713495656947 9 0.053233963268565346 0.11398537187727575 >10 0.46579717859994674 2.534063684049436 >50 0.04879779966285157 0.8348373069900473 >100 0.039925472451424006 1.490253195284383 >500 0.0 0.0 >1k 0.0044361636057137785 3.1736482706941493 >5k 0.0 0.0 >10k+ 0.0044361636057137785 65.28090006226978 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 61853 65.28090006226978 No Hit CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG 3007 3.1736482706941493 No Hit TCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGT 359 0.3788958194809444 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 189 0.19947440078523257 No Hit CTCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG 155 0.1635901170460902 No Hit ATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTGGTC 129 0.13614919418674604 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 126 0.13298293385682172 No Hit GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 123 0.12981667352689738 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 118 0.12453957297702349 No Hit GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 110 0.1160962120972253 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 103 0.10870827132740186 No Hit TCTTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG 95 0.10026491044760368 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.006332520659848653 0.0 5 0.0 0.0 0.0 0.015831301649621633 0.0 6 0.0 0.0 0.0 0.015831301649621633 0.0 7 0.0 0.0 0.0 0.016886721759596407 0.0 8 0.0 0.0 0.0 0.016886721759596407 0.0 9 0.0 0.0 0.0 0.017942141869571184 0.0 10 0.0 0.0 0.0 0.017942141869571184 0.0 11 0.0 0.0 0.0 0.022163822309470286 0.0 12 0.0 0.0 0.0 0.022163822309470286 0.0 13 0.0 0.0 0.0 0.02533008263939461 0.0 14 0.0 0.0 0.0 0.02744092285934416 0.0 15 0.0 0.0 0.0 0.02849634296931894 0.0 16 0.0 0.0 0.0 0.02849634296931894 0.0 17 0.0 0.0 0.0 0.02849634296931894 0.0 18 0.0 0.0 0.0 0.029551763079293712 0.0 19 0.0 0.0 0.0 0.041161384289016244 0.0 20 0.0 0.0 0.0 0.04221680439899102 0.0 21 0.0 0.0 0.0 0.04221680439899102 0.0 22 0.0 0.0 0.0 0.04960474516881445 0.0 23 0.0 0.0 0.0 0.05066016527878922 0.0 24 0.0 0.0 0.0 0.05066016527878922 0.0 25 0.0 0.0 0.0 0.05066016527878922 0.0 26 0.0 0.0 0.0 0.051715585388763995 0.0 27 0.0 0.0 0.0 0.06543604681843608 0.0 28 0.0 0.0 0.0 0.08021192835808294 0.0 29 0.0 0.0 0.0 0.09498780989772979 0.0 30 0.0 0.0 0.0 0.11187453165732619 0.0 31 0.0 0.0 0.0 0.1203178925371244 0.0 32 0.0 0.0 0.0 0.13403835396679648 0.0 33 0.0 0.0 0.0 0.14881423550644335 0.0 34 0.0 0.0 0.0 0.1804768388056866 0.0 35 0.0 0.0 0.0 0.21425028232487942 0.0 36 0.0 0.0 0.0 0.26702128782361817 0.0 37 0.0 0.0 0.0 0.35567657706149935 0.0 38 0.0 0.0 0.0 0.42638972442980927 0.0 39 0.0 0.0 0.0 0.5424859365270346 0.0 40 0.0 0.0 0.0 0.6659700893940833 0.0 41 0.0 0.0 0.0 0.7704566802815861 0.0 42 0.0 0.0 0.0 0.9329913772177015 0.0 43 0.0 0.0 0.0 1.0744176719543215 0.0 44 0.0 0.0 0.0 1.236952368890437 0.0 45 0.0 0.0 0.0 1.3646582021973848 0.0 46 0.0 0.0 0.0 1.513472437703828 0.0 47 0.0 0.0 0.0 1.678117974859893 0.0 48 0.0 0.0 0.0 1.8395972516860337 0.0 49 0.0 0.0 0.0 1.983134386642603 0.0 50 0.0 0.0 0.0 2.0749559362104084 0.0 51 0.0 0.0 0.0 2.170999166218113 0.0 52 0.0 0.0 0.0 2.271264076665717 0.0 53 0.0 0.0 0.0 2.3820831882130684 0.0 54 0.0 0.0 0.0 2.4929022997604195 0.0 55 0.0 0.0 0.0 2.6111093520775945 0.0 56 0.0 0.0 0.0 2.7314272446147188 0.0 57 0.0 0.0 0.0 2.8190271137426253 0.0 58 0.0 0.0 0.0 2.9129595035303804 0.0 59 0.0 0.0 0.0 3.0332773960675046 0.0 60 0.0 0.0 0.0 3.111378484205638 0.0 61 0.0 0.0 0.0 3.2116433946532417 0.0 62 0.0 0.0 0.0 3.2865782224614506 0.0 63 0.0 0.0 0.0 3.341460068180139 0.0 64 0.0 0.0 0.0 3.469165901487087 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTACG 15 0.0022124134 70.04916 2 CTGATGG 15 0.0022124134 70.04916 52 CGAATCA 15 0.0022124134 70.04916 56 CGCGGTA 15 0.0022124134 70.04916 53 GCCGAAT 15 0.0022124134 70.04916 54 GTTGCCA 15 0.0022124134 70.04916 1 CCGAATC 15 0.0022124134 70.04916 55 TTATACG 20 7.86374E-5 70.04915 2 ACAGACG 15 0.002217058 70.01215 11 TTTGGGG 20 7.884368E-5 70.012146 3 CAACAGA 15 0.00222171 69.97519 9 GGTAGGT 15 0.00222171 69.97519 37 AAACGTC 15 0.00222171 69.97519 6 TTTGCGG 15 0.00222171 69.97519 31 GGTCGGC 15 0.00222171 69.97519 50 GGGCGCT 35 2.943143E-7 60.010414 13 ACTCGGA 510 0.0 58.998684 29 CCGTATC 515 0.0 58.487644 55 GCCGTAT 515 0.0 58.487644 54 CGTATCA 510 0.0 58.374294 56 >>END_MODULE