FastQCFastQC Report
Wed 25 May 2016
SRR1780825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94749
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3596337.95607341502285No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG57746.093995714994353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50205.298208952073373No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT22242.3472543245839006No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT19192.0253511910415942No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC16901.7836599858573707No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT7530.7947313428110059No Hit
TCATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG6810.7187410948928221No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5810.6131990838953445No Hit
CTTATACACATCTCCGCGCCCACGAGACCCAAATAAATCTCGTATGCCGT5800.6121436637853698No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT5620.5931461018058238No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5470.5773148001562022No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT4700.4960474516881445No Hit
ATAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2920.3081826721126344No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAACTCTCGTATGCCGT2740.28918511013308845No Hit
CAAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2150.22691532364457673No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAAAAAATCTCGTATGCCGT2110.2226936432046776No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC2100.22163822309470288No Hit
CTAAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT1900.20052982089520735No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCAAATAAATCTCGTATGCCGT1880.1984189806752578No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1840.19419730023535867No Hit
AATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT1660.17519973825581273No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGACGT1600.16886721759596407No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAAATAAATCTCGTATGCCGT1470.15514675616629198No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1420.14986965561641813No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1350.1424817148465947No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATATCGTATGCCGT1340.1414262947366199No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCAGT1280.13509377407677126No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC1280.13509377407677126No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC1180.12453957297702349No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1080.11398537187727575No Hit
TCTTATACACATCTCCGCGCCCACGAGACCCAAATAAATCTCGTATGCCG1050.11081911154735144No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1040.10976369143737665No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT1040.10976369143737665No Hit
ATTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT1020.10765285121742708No Hit
CTTATACACATCTCCGAGACCACGAGACCCAAATAAATCTCGTATGCCGT1000.10554201099747754No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAAATAAATCTCGTATGCCGT960.10132033055757844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGAA150.00221214970.0512752
AGCGGGG207.862564E-570.0512770
TCTGAAT150.00221214970.0512753
CTACTGA150.00221214970.0512751
AAGCATG150.00221214970.0512754
CGTCATA150.002216793170.0142748
GAGAAAC150.002216793170.0142724
CTATACA150.002216793170.014271
TTCTTGA150.002216793170.0142755
AGCACCA150.002216793170.0142725
CGACTTA150.002216793170.0142748
CGTCTAA207.883189E-570.0142748
CGACATC207.883189E-570.0142748
ATCTAAG207.903857E-569.97729510
AACTCAG207.903857E-569.97729510
TAACCGA150.002221444669.97729511
TCTAAGA207.903857E-569.97729511
TACGAGA150.002221444669.97729513
GCAGACA150.002221444669.97729546
GAACACG207.903857E-569.97729518
Produced by FastQC (version 0.11.5)