FastQCFastQC Report
Wed 25 May 2016
SRR1780824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88288
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5826965.99877673069953No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG28793.260918811163465No Hit
TCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGT3360.38057267125770206No Hit
CTCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG1410.1597046031170714No Hit
ATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTGGTC1190.13478615440376948No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG990.11213301920985864No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG940.10646973541138094No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG900.10193910837259876No Hit
TCTTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG890.10080645161290322No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCT150.00221858469.9960413
GCCGGCG252.3795855E-455.99682620
TAAAGGT252.3795855E-455.99682643
AAAAAGG2550.054.8988570
GTAGATC4550.054.64325338
AGTGTAG4550.053.87362735
GATCTCG4550.053.87362741
GTATCAT4300.053.71788857
CATTAAA4300.053.71788861
GTGTAGA4500.053.6940536
TAGATCT4500.053.6940539
ATCTCGG4500.053.66362842
TCTCGGT4500.053.66362843
CTCGGTG4500.053.66362844
ATCATTA4250.053.5263859
CGTATCA4450.053.48011856
TCGGAGT4200.053.3303131
GAGTGTA4400.053.29243534
GTCGCCG4400.053.29243551
TCGGTGG4400.053.29243545