##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780824_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88288 Sequences flagged as poor quality 0 Sequence length 76 %GC 87 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.200423613628125 32.0 27.0 32.0 13.0 32.0 2 28.96101395433128 32.0 32.0 32.0 13.0 32.0 3 28.959360275462124 32.0 32.0 32.0 13.0 32.0 4 28.439969191736136 32.0 27.0 32.0 13.0 32.0 5 28.97414144617615 32.0 32.0 32.0 13.0 32.0 6 33.038940739398335 37.0 32.0 37.0 13.0 37.0 7 32.595743475897066 37.0 32.0 37.0 13.0 37.0 8 32.98635148604567 37.0 32.0 37.0 13.0 37.0 9 33.122712033345415 37.0 37.0 37.0 13.0 37.0 10-11 32.88519957412106 37.0 32.0 37.0 13.0 37.0 12-13 32.62152840703153 37.0 32.0 37.0 13.0 37.0 14-15 33.13971321130845 37.0 34.5 37.0 17.5 37.0 16-17 33.41420691373686 37.0 34.5 37.0 17.5 37.0 18-19 33.00205577201885 37.0 32.0 37.0 17.5 37.0 20-21 33.38550539144617 37.0 34.5 37.0 17.5 37.0 22-23 33.41601350126857 37.0 34.5 37.0 17.5 37.0 24-25 32.95328357194636 37.0 32.0 37.0 13.0 37.0 26-27 33.40890041681769 37.0 34.5 37.0 17.5 37.0 28-29 33.22977075027184 37.0 34.5 37.0 13.0 37.0 30-31 33.2068571040232 37.0 37.0 37.0 13.0 37.0 32-33 33.32716790503805 37.0 37.0 37.0 17.5 37.0 34-35 33.16711217832548 37.0 34.5 37.0 13.0 37.0 36-37 33.827949438202246 37.0 37.0 37.0 24.5 37.0 38-39 33.92428189561436 37.0 37.0 37.0 24.5 37.0 40-41 33.418896112721995 37.0 34.5 37.0 17.5 37.0 42-43 33.26815082457412 37.0 37.0 37.0 17.5 37.0 44-45 33.12659138274737 37.0 34.5 37.0 13.0 37.0 46-47 33.35172390358826 37.0 34.5 37.0 17.5 37.0 48-49 33.81096525009062 37.0 37.0 37.0 24.5 37.0 50-51 34.04267284342153 37.0 37.0 37.0 24.5 37.0 52-53 33.35848586444364 37.0 34.5 37.0 17.5 37.0 54-55 33.50808150598043 37.0 37.0 37.0 17.5 37.0 56-57 33.37319907575208 37.0 37.0 37.0 17.5 37.0 58-59 32.98393326386372 37.0 34.5 37.0 13.0 37.0 60-61 32.82891219644799 37.0 34.5 37.0 13.0 37.0 62-63 32.47537604204422 37.0 32.0 37.0 13.0 37.0 64-65 32.17777047843422 37.0 32.0 37.0 13.0 37.0 66-67 32.136966518666185 37.0 32.0 37.0 13.0 37.0 68-69 32.42681338347227 37.0 32.0 37.0 13.0 37.0 70-71 32.68880821855745 37.0 32.0 37.0 13.0 37.0 72-73 33.04064538782167 37.0 32.0 37.0 13.0 37.0 74-75 32.945547526277636 37.0 32.0 37.0 13.0 37.0 76 33.47094735411381 37.0 37.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.3786686818077989 11101 2 0.47968345197031326 11101 3 -0.75208579544298 11101 4 -0.24755365659154194 11101 5 -0.13405986609247833 11101 6 0.8822045279214876 11101 7 1.30360924902255 11101 8 -1.437023603922075 11101 9 0.1560253981459283 11101 10-11 -1.230302120065467 11101 12-13 0.5306459062419364 11101 14-15 -0.09763679624111887 11101 16-17 0.4357513908570567 11101 18-19 0.30895088760712497 11101 20-21 0.9335936799406355 11101 22-23 -0.7370040301331997 11101 24-25 -0.0013560100707152856 11101 26-27 0.3458297087895943 11101 28-29 -0.4944915203460596 11101 30-31 -0.9861717570936648 11101 32-33 0.2190627422849829 11101 34-35 -1.6858055892828077 11101 36-37 -1.1108793485023511 11101 38-39 -0.7390342976199449 11101 40-41 -0.6462714864726351 11101 42-43 -0.29369411994725425 11101 44-45 0.6938566635655476 11101 46-47 0.1445267531285097 11101 48-49 0.9625867255236997 11101 50-51 -0.14655661339347148 11101 52-53 -0.3612637144308266 11101 54-55 0.052241773190885965 11101 56-57 0.23716721256291606 11101 58-59 -0.7139141380402734 11101 60-61 0.8460634873220982 11101 62-63 0.18738897701829416 11101 64-65 -1.672435718552137 11101 66-67 0.03103975755268351 11101 68-69 -0.5173416085831803 11101 70-71 -0.5532863297855499 11101 72-73 -1.6440525534197334 11101 74-75 -0.6419681240913846 11101 76 0.3231589543799771 11102 1 0.9479722653101632 11102 2 -1.626469353979651 11102 3 -0.6449187907100509 11102 4 -1.9091493293433999 11102 5 -1.3189821108524526 11102 6 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0.4568799581130669 11105 6 1.118174778090527 11105 7 1.8374158751212661 11105 8 1.973050770655341 11105 9 1.6651531871925798 11105 10-11 1.7781495364592104 11105 12-13 0.705595196302788 11105 14-15 -0.8226198929280741 11105 16-17 0.93304686076916 11105 18-19 0.9058068713799514 11105 20-21 0.027745133625565188 11105 22-23 0.6237126703400904 11105 24-25 1.1702126173802654 11105 26-27 0.2966410678159619 11105 28-29 0.6867119955430638 11105 30-31 0.4230574518312764 11105 32-33 -8.4936048715889E-4 11105 34-35 0.7452288934758116 11105 36-37 0.6781673046416614 11105 38-39 1.1142449451116292 11105 40-41 0.5643437941223581 11105 42-43 1.225913723189997 11105 44-45 1.4110642362497927 11105 46-47 0.47955041776678087 11105 48-49 0.6904433854290417 11105 50-51 0.3433013987769158 11105 52-53 1.1155787466915612 11105 54-55 0.7892262221428794 11105 56-57 0.2958217088441941 11105 58-59 -0.654245442976034 11105 60-61 0.46692217562500815 11105 62-63 0.595096887768797 11105 64-65 0.04223635717470131 11105 66-67 0.5038254235432191 11105 68-69 1.061427830226826 11105 70-71 0.2913722232908498 11105 72-73 0.9986114087168403 11105 74-75 -0.23037245133952666 11105 76 -2.0372196798323294 11106 1 -0.6981554904840692 11106 2 0.028703059813452114 11106 3 0.12415978475631206 11106 4 0.7203023382654195 11106 5 0.1346640169033435 11106 6 0.14417816983727505 11106 7 0.06381497065547137 11106 8 0.6867308158786329 11106 9 0.06537930364255118 11106 10-11 -0.7656605257228151 11106 12-13 0.23315313864309672 11106 14-15 -0.27442882452784545 11106 16-17 0.3290332937821603 11106 18-19 -0.38150234286538875 11106 20-21 -0.46193830334448194 11106 22-23 0.3899647901818071 11106 24-25 0.018573273117972633 11106 26-27 -0.4188761735633477 11106 28-29 -0.016510163869043026 11106 30-31 -0.9982257590223114 11106 32-33 -0.019927935953525377 11106 34-35 0.740423918913919 11106 36-37 -0.5250548547704383 11106 38-39 0.44691877213254116 11106 40-41 -0.43396033250284916 11106 42-43 0.4636186412227872 11106 44-45 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0.40326179410458707 21611 32-33 0.14825664206711764 21611 34-35 0.4253055218283066 21611 36-37 0.805242655854947 21611 38-39 0.0823164649072865 21611 40-41 -0.13265492873843243 21611 42-43 0.5777655750418518 21611 44-45 1.4433120012561815 21611 46-47 -0.4763882795512302 21611 48-49 1.0317588388134666 21611 50-51 -0.3192145654631844 21611 52-53 -1.0769499889406333 21611 54-55 0.4073615988989445 21611 56-57 1.7191933563537631 21611 58-59 -0.1229555323757765 21611 60-61 0.7376756877578288 21611 62-63 0.5152756872579474 21611 64-65 0.008392168158103175 21611 66-67 0.3240680799927702 21611 68-69 1.1961660166891477 21611 70-71 0.4162125808898267 21611 72-73 0.9692372069288524 21611 74-75 0.9615177146885685 21611 76 1.8503920698483896 21612 1 -0.30316631634240565 21612 2 -0.5562026005639069 21612 3 -0.44095002037823505 21612 4 -0.44363208796409026 21612 5 -0.35899512321419635 21612 6 -2.0008883318639334 21612 7 -0.14404150939402172 21612 8 -1.7670273035151212 21612 9 0.48232950469420643 21612 10-11 0.10118141674548298 21612 12-13 0.35084568426635343 21612 14-15 -0.7609738291674972 21612 16-17 -0.5529648503564104 21612 18-19 -1.272625139029941 21612 20-21 -0.07066085206084693 21612 22-23 0.028071811524213786 21612 24-25 -1.3439383260159623 21612 26-27 -0.9094422112991936 21612 28-29 -0.8071071326287012 21612 30-31 -1.064418149990452 21612 32-33 0.3093328125772459 21612 34-35 -1.261277287396016 21612 36-37 -1.183576351831988 21612 38-39 -0.9358526476014504 21612 40-41 -1.2124942019739322 21612 42-43 -1.104274956055285 21612 44-45 -0.7813560630345293 21612 46-47 -0.6860318854212437 21612 48-49 -0.6791076880908022 21612 50-51 0.14811597262206533 21612 52-53 0.01424497961283322 21612 54-55 -0.3952240055734535 21612 56-57 -0.9951672306483275 21612 58-59 -1.1332630096643932 21612 60-61 -0.43825023816809505 21612 62-63 -1.7565621883535165 21612 64-65 -1.062187845818137 21612 66-67 -0.4302994951295247 21612 68-69 -1.3094244794673884 21612 70-71 -0.08972732127648442 21612 72-73 -1.4255915841263551 21612 74-75 0.07297823180946494 21612 76 0.36681415230820136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 9.0 12 31.0 13 94.0 14 222.0 15 316.0 16 506.0 17 713.0 18 815.0 19 885.0 20 889.0 21 1043.0 22 1027.0 23 1185.0 24 1316.0 25 1418.0 26 1603.0 27 1836.0 28 1989.0 29 2290.0 30 2817.0 31 3272.0 32 4207.0 33 5766.0 34 8220.0 35 14105.0 36 31712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 82.88044401653735 3.888542787563006 2.8407996828453306 10.390213513054313 2 79.37135413716939 4.584017670045874 10.737950954295746 5.306677238488985 3 78.78544161125522 4.53901833958246 11.583728859638192 5.0918111895241225 4 78.481543149048 8.636605615775823 7.288728804920316 5.59312243025587 5 78.11682354140473 6.886631100841574 11.959858189767463 3.036687167986227 6 81.26543279795212 11.722130349092721 4.160342523163355 2.852094329791813 7 80.21951882560826 6.64559829640705 4.141180734901001 8.993702143083684 8 80.73668830065581 11.190775538867557 3.7649937136837814 4.307542446792846 9 79.01729586462332 4.630353279644795 3.753667017794239 12.598683837937635 10-11 80.4532995038851 7.6994087397775415 7.151756790431099 4.695534965906259 12-13 79.82964359945858 5.099365127908887 7.648481347446637 7.422509925185902 14-15 83.03609353850337 6.645560659005828 4.836636102600117 5.481709699890694 16-17 82.54573257065186 5.601178003058277 4.349549753638784 7.5035396726510735 18-19 79.64863395405736 5.284083186081283 7.258959720900729 7.808323138960627 20-21 82.66636461460044 5.146400860848389 5.055218893356742 7.132015631194427 22-23 82.46474486039531 5.086934360310359 4.442430764002945 8.005890015291387 24-25 80.57290752266768 7.769591047328866 4.2724538859282 7.385047544075254 26-27 82.88922995021889 7.663119502981769 4.511448522707322 4.936202024092019 28-29 80.61039564148741 10.29086955044344 4.743620238540215 4.355114569528923 30-31 80.40436075322101 5.848506300438907 6.846382556987115 6.900750389352966 32-33 82.3102321445764 5.248313709499295 5.291355885168006 7.150098260756296 34-35 83.34371637310981 7.207339865209265 4.917596420683015 4.531347340997904 36-37 83.20203433218741 5.512292644801748 7.314987342058912 3.970685680951923 38-39 83.13813218553548 5.016707254913066 7.973041853089426 3.8721187064620266 40-41 82.73875935186811 7.300828571266757 5.717311645872152 4.243100430992983 42-43 80.74313610003625 7.508947988401594 7.5185755708590065 4.229340340703153 44-45 80.00453078099338 4.9821600498385905 8.10273545902475 6.910573710143286 46-47 82.24926800813262 4.645670629144886 5.649787906418308 7.455273456304192 48-49 83.11500756069796 4.507535212464108 7.852365337456321 4.525091889381608 50-51 85.19810615499627 4.466166776159301 5.891081258636703 4.444645810207734 52-53 80.26425331166148 4.93903371410125 7.851710057596575 6.94500291664071 54-55 82.21199044026866 4.80535073113822 5.413027965612152 7.56963086298096 56-57 82.0987829120627 5.017868368739699 4.975958407194922 7.907390312002673 58-59 80.23301256817912 7.36079566372333 7.5494033088464345 4.85678845925112 60-61 79.47579457671658 5.599415535872052 7.9287769295245 6.996012957886867 62-63 79.65034291766013 7.6987987970980845 7.4246912042044935 5.226167081037305 64-65 79.55506974689494 7.762492424971825 8.00602604110621 4.676411787027021 66-67 79.53423043813149 10.813964025191428 4.836663495084046 4.815142041593041 68-69 79.70233106041728 11.033459438642593 4.3851799832363 4.8790295177038265 70-71 79.88321184419938 11.119795649046495 4.308474787464813 4.688517719289303 72-73 80.57177484666398 10.429114303998913 4.25433106986912 4.744779779467982 74-75 82.24267308509133 8.82967577516636 4.233894945490585 4.693756194251734 76 85.89826361752013 5.27257693658183 4.212398201320693 4.6167612445773445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 0.5 10 0.5 11 0.5 12 0.0 13 1.0 14 2.0 15 3.5 16 4.5 17 4.0 18 8.5 19 11.5 20 12.0 21 14.0 22 23.5 23 34.0 24 55.5 25 76.0 26 67.5 27 75.0 28 122.5 29 154.0 30 174.0 31 195.0 32 231.0 33 266.0 34 290.5 35 363.0 36 429.5 37 448.0 38 513.5 39 626.0 40 764.5 41 890.0 42 924.0 43 1096.0 44 1972.0 45 2869.0 46 3062.0 47 2180.0 48 1167.5 49 935.5 50 834.0 51 754.5 52 669.0 53 593.0 54 523.0 55 507.5 56 435.5 57 353.0 58 327.0 59 283.5 60 238.0 61 216.0 62 196.0 63 183.5 64 155.0 65 130.5 66 110.5 67 99.0 68 91.5 69 79.0 70 78.0 71 82.0 72 90.0 73 95.5 74 86.0 75 79.0 76 83.5 77 94.0 78 104.0 79 108.0 80 102.0 81 107.5 82 129.5 83 140.0 84 153.0 85 171.5 86 180.5 87 184.0 88 202.5 89 244.0 90 277.0 91 341.5 92 396.0 93 447.0 94 569.0 95 761.0 96 882.0 97 1113.0 98 2044.0 99 31132.5 100 59521.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033979702790866255 2 0.0033979702790866255 3 0.010193910837259876 4 0.0011326567596955418 5 0.0011326567596955418 6 0.0022653135193910837 7 0.004530627038782167 8 0.0011326567596955418 9 0.0011326567596955418 10-11 0.0022653135193910837 12-13 0.0028316418992388546 14-15 0.003964298658934396 16-17 0.0033979702790866255 18-19 0.004530627038782167 20-21 0.0033979702790866255 22-23 0.0033979702790866255 24-25 0.0016989851395433127 26-27 0.0016989851395433127 28-29 0.0011326567596955418 30-31 5.663283798477709E-4 32-33 0.0028316418992388546 34-35 0.0033979702790866255 36-37 0.003964298658934396 38-39 0.0033979702790866255 40-41 0.003964298658934396 42-43 0.0 44-45 0.0033979702790866255 46-47 0.0016989851395433127 48-49 0.0028316418992388546 50-51 0.0022653135193910837 52-53 0.0016989851395433127 54-55 0.0011326567596955418 56-57 0.003964298658934396 58-59 0.010760239217107647 60-61 0.0022653135193910837 62-63 0.0016989851395433127 64-65 0.005096955418629938 66-67 0.004530627038782167 68-69 0.0022653135193910837 70-71 0.009627582457412106 72-73 0.0016989851395433127 74-75 5.663283798477709E-4 76 0.0011326567596955418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 88288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.334722725625227 #Duplication Level Percentage of deduplicated Percentage of total 1 87.40178381822042 18.646928234867705 2 5.749628371204078 2.453334541500544 3 2.9305585049904437 1.8756795940558173 4 1.6245487364620936 1.3863718738673432 5 0.7804204714376726 0.8325027183762234 6 0.3981737099171798 0.5096955418629938 7 0.29199405393926525 0.4360728524827836 8 0.07963474198343598 0.13591881116346502 9 0.07963474198343598 0.15290866255889815 >10 0.5627521766829475 2.4657937658571947 >50 0.07432575918454025 1.1700344327654948 >100 0.015926948396687193 0.675063428778543 >500 0.0 0.0 >1k 0.005308982798895732 3.260918811163465 >5k 0.0 0.0 >10k+ 0.005308982798895732 65.99877673069953 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 58269 65.99877673069953 No Hit CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG 2879 3.260918811163465 No Hit TCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGT 336 0.38057267125770206 No Hit CTCTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG 141 0.1597046031170714 No Hit ATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTGGTC 119 0.13478615440376948 No Hit GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 99 0.11213301920985864 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 94 0.10646973541138094 No Hit GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 90 0.10193910837259876 No Hit TCTTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGG 89 0.10080645161290322 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0022653135193910837 0.0 2 0.0 0.0 0.0 0.0022653135193910837 0.0 3 0.0 0.0 0.0 0.0022653135193910837 0.0 4 0.0 0.0 0.0 0.007928597317868793 0.0 5 0.0 0.0 0.0 0.01245922435665096 0.0 6 0.0 0.0 0.0 0.013591881116346502 0.0 7 0.0 0.0 0.0 0.013591881116346502 0.0 8 0.0 0.0 0.0 0.016989851395433127 0.0 9 0.0 0.0 0.0 0.016989851395433127 0.0 10 0.0 0.0 0.0 0.016989851395433127 0.0 11 0.0 0.0 0.0 0.01812250815512867 0.0 12 0.0 0.0 0.0 0.020387821674519753 0.0 13 0.0 0.0 0.0 0.020387821674519753 0.0 14 0.0 0.0 0.0 0.020387821674519753 0.0 15 0.0 0.0 0.0 0.020387821674519753 0.0 16 0.0 0.0 0.0 0.020387821674519753 0.0 17 0.0 0.0 0.0 0.020387821674519753 0.0 18 0.0 0.0 0.0 0.021520478434215295 0.0 19 0.0 0.0 0.0 0.026051105472997462 0.0 20 0.0 0.0 0.0 0.027183762232693004 0.0 21 0.0 0.0 0.0 0.027183762232693004 0.0 22 0.0 0.0 0.0 0.0351123595505618 0.0 23 0.0 0.0 0.0 0.0351123595505618 0.0 24 0.0 0.0 0.0 0.04190830010873505 0.0 25 0.0 0.0 0.0 0.044173613628126135 0.0 26 0.0 0.0 0.0 0.044173613628126135 0.0 27 0.0 0.0 0.0 0.05550018122508155 0.0 28 0.0 0.0 0.0 0.06795940558173251 0.0 29 0.0 0.0 0.0 0.08721457049655672 0.0 30 0.0 0.0 0.0 0.09967379485320768 0.0 31 0.0 0.0 0.0 0.12232693004711852 0.0 32 0.0 0.0 0.0 0.1461127220007249 0.0 33 0.0 0.0 0.0 0.16989851395433128 0.0 34 0.0 0.0 0.0 0.21407212758245742 0.0 35 0.0 0.0 0.0 0.26390902500906127 0.0 36 0.0 0.0 0.0 0.3194092062341428 0.0 37 0.0 0.0 0.0 0.4462667633200435 0.0 38 0.0 0.0 0.0 0.566328379847771 0.0 39 0.0 0.0 0.0 0.7350942370424066 0.0 40 0.0 0.0 0.0 0.9038600942370424 0.0 41 0.0 0.0 0.0 1.122462848858282 0.0 42 0.0 0.0 0.0 1.3682493657122146 0.0 43 0.0 0.0 0.0 1.5981786879304096 0.0 44 0.0 0.0 0.0 1.8383019209858644 0.0 45 0.0 0.0 0.0 2.103343602754621 0.0 46 0.0 0.0 0.0 2.359324030445814 0.0 47 0.0 0.0 0.0 2.6028452337803554 0.0 48 0.0 0.0 0.0 2.7693457774556 0.0 49 0.0 0.0 0.0 2.9596321130844507 0.0 50 0.0 0.0 0.0 3.1657756433490394 0.0 51 0.0 0.0 0.0 3.3639905762957594 0.0 52 0.0 0.0 0.0 3.5463483146067416 0.0 53 0.0 0.0 0.0 3.698124320405944 0.0 54 0.0 0.0 0.0 3.8431043856469733 0.0 55 0.0 0.0 0.0 3.984686480608916 0.0 56 0.0 0.0 0.0 4.130799202609642 0.0 57 0.0 0.0 0.0 4.295034432765495 0.0 58 0.0 0.0 0.0 4.437749184487133 0.0 59 0.0 0.0 0.0 4.562341428053642 0.0 60 0.0 0.0 0.0 4.708454150054368 0.0 61 0.0 0.0 0.0 4.861362812613265 0.0 62 0.0 0.0 0.0 5.06863899963755 0.0 63 0.0 0.0 0.0 5.214751721638275 0.0 64 0.0 0.0 0.0 5.358599130119608 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGCT 15 0.002218584 69.99604 13 GCCGGCG 25 2.3795855E-4 55.996826 20 TAAAGGT 25 2.3795855E-4 55.996826 43 AAAAAGG 255 0.0 54.89885 70 GTAGATC 455 0.0 54.643253 38 AGTGTAG 455 0.0 53.873627 35 GATCTCG 455 0.0 53.873627 41 GTATCAT 430 0.0 53.717888 57 CATTAAA 430 0.0 53.717888 61 GTGTAGA 450 0.0 53.69405 36 TAGATCT 450 0.0 53.69405 39 ATCTCGG 450 0.0 53.663628 42 TCTCGGT 450 0.0 53.663628 43 CTCGGTG 450 0.0 53.663628 44 ATCATTA 425 0.0 53.52638 59 CGTATCA 445 0.0 53.480118 56 TCGGAGT 420 0.0 53.33031 31 GAGTGTA 440 0.0 53.292435 34 GTCGCCG 440 0.0 53.292435 51 TCGGTGG 440 0.0 53.292435 45 >>END_MODULE