FastQCFastQC Report
Wed 25 May 2016
SRR1780824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88288
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3443038.99737223631751No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG52425.93738673432403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48285.4684668358100765No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC18052.044445451250453No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT17962.034251540413193No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT14231.611770569046756No Hit
TCATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG5300.6003080826386372No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC5160.5844508880028996No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4870.5516038419717288No Hit
CTTATACACATCTCCGCGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4700.5323486770569047No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4100.46438927147517217No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT3660.4145523740485683No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT3480.39416455237404857TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT2830.32054186299383836No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT2240.25371511417180137No Hit
ATAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1840.2084088437839797No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT1790.20274555998550198No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT1590.18009242479159115No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1580.17895976803189562No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT1580.17895976803189562No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1540.17442914099311344No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGGTCATCTCGTATGCCGT1460.1653678869155491No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGACATCTCGTATGCCGT1420.16083725987676695No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1370.15517397607828923No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT1300.14724537876042043No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC1280.14498006524102935No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGA1240.1404494382022472No Hit
CTAAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1180.13365349764407394No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1170.1325208408843784No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC1170.1325208408843784No Hit
CTTATACACATCTCCGAGCCCACGAGCCCTTGGGTCATCTCGTATGCCGT1110.12572490032620515No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT1000.11326567596955418No Hit
AATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT960.10873504893077202No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG910.10307176513229431No Hit
CAAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT910.10307176513229431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGGG252.8193026E-670.0000128
CTGAATG500.070.0000154
TGCTTTA252.8193026E-670.0000155
GACGTAT252.8193026E-670.0000146
ATGCCCT252.8193026E-670.0000144
GGGACAT650.070.0000132
ATCCCAC150.002218085870.017
ACACGTA150.002218085870.038
AACGTAT207.88819E-570.039
ATCTTAT150.002218085870.01
TGCGTGA150.002218085870.055
CAGTATT150.002218085870.047
ACGTCAT353.6616257E-970.047
AAACAAC150.002218085870.06
TATGCTA150.002218085870.053
AGTGGGG150.002218085870.070
CCTTGTG150.002218085870.028
TTCTGAA301.013359E-770.052
GGTTCAT150.002218085870.032
CAACACG207.88819E-570.036
Produced by FastQC (version 0.11.5)