FastQCFastQC Report
Wed 25 May 2016
SRR1780823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545579
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6288411.526103460727043No Hit
CTTATACACATCTGACGCTGCCGACGAAACTCGGAGTGTAGATCTCGGTG31460.5766350977585281No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGA200.00695145852.49738324
TACGCGT200.00695397852.4925735
TTGCGCG200.00695397852.4925739
GCCGTAT5300.052.17199754
CTCGGTG5600.051.8819344
GTCGCCG5650.050.79858851
TGGTCGC5600.050.62248249
CGGTGGT5850.050.2585246
TCGGTGG5800.050.08830645
TCGCCGT5400.049.909552
GATCTCG6050.047.44435541
TCTCGGT6250.047.04628443
TAGATCT6200.046.86110339
AGATCTC6200.046.29226340
GTGGTCG6350.045.74575448
ATCTCGG6300.045.5616442
GTAGATC6350.045.2028938
GGTGGTC6350.045.19874647
CGCCGTA6150.044.96123553
CGTATCA6250.043.6818356
Produced by FastQC (version 0.11.5)