FastQCFastQC Report
Wed 25 May 2016
SRR1780823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545579
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT376636.903308228505862No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG58311.0687728083375643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52060.9542156131376024No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT21920.40177499500530633No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT20630.37813038991603415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC19340.354485784826762No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT8210.15048233161466992No Hit
TCATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG7490.13728534272763432No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC6510.1193227745202803No Hit
CTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT6140.11254098856444254No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6120.11217440553980267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATT207.925865E-569.9982547
AGGGGGG51950.064.075570
TGCCGTA850.061.76317245
GCCGTAT650.059.22929846
TATGCCG68500.054.8745543
GCCGTCT66300.054.84780546
CGTCTTC66850.054.65832548
CGTATGC70000.054.6486441
CCGTCTT66100.054.64311647
TCGTATG71100.054.59076740
ATGCCGT68600.054.5904844
ATCTCGT70450.054.4982937
CTCGTAT70450.054.4982939
TGCCGTC68150.054.48874345
TCTCGTA70900.054.34984638
GTATGCC69250.054.22969442
AATCTCG71250.054.18110336
ATATCGT1100.054.0895637
TCTTCTG67550.053.94142550
CTTGAAA69700.053.8293657