FastQCFastQC Report
Wed 25 May 2016
SRR1780822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599844
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291564.860597088576363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG200.006950948452.49911
CGTCGCT200.006953240452.49462518
CGGTGGT1500.051.3366446
GCCGTAT1200.049.59065654
TCGCCGT1400.047.50701552
GTCGCCG1400.047.50305651
TCGGTGG1600.045.94045345
TGGTCGC1550.045.15666649
CGACGTT404.5926827E-543.7491714
CTCGGTG1700.043.24168444
GATCTCG1450.041.04054341
CGCCGTA1700.037.064353
GTAGATC1700.037.05811738
TAGATCT1800.036.9437439
GGTCATA601.2143488E-535.005171
CCGACGT501.7097019E-434.99933213
TCGTTGT501.71054E-434.99641434
CATCGTT501.71054E-434.99641432
ATCTCGG1750.034.0050242
TCTCGGT2500.032.20475843
Produced by FastQC (version 0.11.5)